公开(公告)号：US20200224253A1

公开(公告)日：2020-07-16

申请号：US16778618

申请日：2020-01-31

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Masayuki Ogino ,  Yoichi Makino

IPC: C12Q1/6818 ,  C12Q1/6876 ,  C12Q1/6837

Abstract: A method of detecting a nucleic acid including mixing a fluid including a target nucleic acid with a detection reagent including a first enzyme for cleaving a first nucleic acid having a first flap and a second enzyme for cleaving a second nucleic acid such that the target nucleic acid, the first nucleic acid and the second nucleic acid form a complex as a first invasive structure, conducting a first reaction which causes the first enzyme to cleave the first flap of the first invasive structure and produces a third nucleic acid that forms a complex, as a second invasive structure, with a fourth nucleic acid having a second flap, and conducting a second reaction which causes the second enzyme to cleave the second flap of the second invasive structure and produces a cleaved product.

公开(公告)号：US12173354B2

公开(公告)日：2024-12-24

申请号：US16778618

申请日：2020-01-31

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Masayuki Ogino ,  Yoichi Makino

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6837 ,  C12Q1/6876

Abstract: A method of detecting a nucleic acid including mixing a fluid including a target nucleic acid with a detection reagent including a first enzyme for cleaving a first nucleic acid having a first flap and a second enzyme for cleaving a second nucleic acid such that the target nucleic acid, the first nucleic acid and the second nucleic acid form a complex as a first invasive structure, conducting a first reaction which causes the first enzyme to cleave the first flap of the first invasive structure and produces a third nucleic acid that forms a complex, as a second invasive structure, with a fourth nucleic acid having a second flap, and conducting a second reaction which causes the second enzyme to cleave the second flap of the second invasive structure and produces a cleaved product.

公开(公告)号：US10364460B2

公开(公告)日：2019-07-30

申请号：US15082759

申请日：2016-03-28

Applicant: TOPPAN PRINTING CO., LTD.

Inventor： Masayuki Ogino ,  Tomohiko Azuma ,  Kenichi Sakane

IPC: C12Q1/68 ,  C12Q1/686 ,  G01N21/64 ,  G01N21/25 ,  G01N21/33

Abstract: A reaction container includes a base material, an analysis well in which a nucleic acid analysis reagent used for a nucleic acid analysis is positioned, the analysis well being arranged on the base material and configured to analyze a sample containing a nucleic acid, and a quantification well in which a quantification reagent configured to specifically detect the nucleic acid is positioned, the quantification well being arranged on the base material and configured to quantify an amount of the nucleic acid contained in the sample, in which the nucleic acid contained in the sample is distributed into the quantification well and the analysis well.