公开(公告)号：US12195787B2

公开(公告)日：2025-01-14

申请号：US17360008

申请日：2021-06-28

Applicant: Government of the United States of America, as represented by the Secretary of Commerce

Inventor： Arvind Kumar Balijepalli ,  Jacob Michael Majikes

IPC: C12Q1/6823 ,  G01N33/53

Abstract: A biomarker signal amplifier amplifies chemical analyte binding and includes: a surface strand disposed on an analysis substrate and including an exchange region; a particle strand hybridized to the surface strand in an absence of a chemical analyte that preferentially hybridizes to the exchange region as compared with the particle strand, and the particle strand is dissociated from the surface strand when the surface strand is in a presence of the chemical analyte; and a reporter particle attached to the particle strand and disposed proximate to the analysis substrate when the particle strand is hybridized to the surface strand in absence of the chemical analyte and that changes the electrical potential of the analysis substrate depending on whether the particle strand is hybridized to the surface strand.

公开(公告)号：US20240417781A1

公开(公告)日：2024-12-19

申请号：US18743591

申请日：2024-06-14

Applicant: Resolve BioSciences GmbH

Inventor： Cheng Frank Zhong ,  Christian Korfhage ,  Frank Reinecke ,  Stephan Tirier

IPC: C12Q1/6823 ,  C12Q1/6804 ,  C12Q1/6876 ,  G01N21/64

Abstract: The technology provided herein relates to high resolution multiplex methods and kits for detecting different analytes in a sample by sequential signal-encoding of said analytes, wherein the method allows a differentiation of targets which distance is below the diffraction limit of optical microscopes, i.e. targets with spatial optical overlap. The disclosed methods also include in vitro methods for screening, identifying and/or testing a substance and/or drug and in vitro methods for diagnosis of a disease, and an optical multiplexing system.

公开(公告)号：US20240401158A1

公开(公告)日：2024-12-05

申请号：US18431664

申请日：2024-02-02

Applicant: Mammoth Biosciences, Inc.

Inventor： Clare Louise FASCHING ,  James Paul BROUGHTON ,  Jesus CHING ,  Janice Sha CHEN ,  Carley Gelenter HENDRIKS ,  Bridget Ann Paine MCKAY

IPC: C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6844

Abstract: Compositions and methods for improved SNP discrimination are provided. An exemplary composition comprises a programmable nuclease and a non-naturally occurring guide nucleic acid that hybridizes to a target nucleic acid, or segment thereof, comprising at least one single-nucleotide polymorphism (SNP). Methods may comprise a) contacting the sample to: i) a detector nucleic acid and ii) the composition; and b) assaying for a signal indicative of cleavage of the detector nucleic acid by the first programmable nuclease. In an exemplary embodiment, the target nucleic acid is a coronavirus target nucleic acid.

公开(公告)号：US20240318233A1

公开(公告)日：2024-09-26

申请号：US18394469

申请日：2023-12-22

Applicant: LUMINEX CORPORATION

Inventor： Douglas F. WHITMAN ,  Hongwei ZHANG

IPC: C12Q1/6818 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

CPC classification number: C12Q1/6818 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

公开(公告)号：US12084711B2

公开(公告)日：2024-09-10

申请号：US17746657

申请日：2022-05-17

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Anusha Dias ,  Michael Heartlein

IPC: C12Q1/68 ,  C07K14/505 ,  C12N9/02 ,  C12Q1/6823

CPC classification number: C12Q1/68 ,  C07K14/505 ,  C12N9/0069 ,  C12Q1/6823 ,  C12Y113/12007 ,  C12Q1/6823 ,  C12Q2521/327 ,  C12Q2525/186 ,  C12Q2537/113 ,  C12Q2565/137

Abstract: The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly for mRNA synthesized in vitro. In some embodiments, the methods comprise chromatographic methods of quantifying capping efficiency and methylation status of the caps.

公开(公告)号：US12077808B2

公开(公告)日：2024-09-03

申请号：US17735509

申请日：2022-05-03

Applicant: The University of North Carolina at Chapel Hill

Inventor： John Michael Ramsey ,  William Henley ,  Emily Oblath

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6823 ,  C12Q1/6853 ,  C12Q1/686 ,  G01N35/00

CPC classification number: C12Q1/6823 ,  B01L3/502761 ,  B01L7/52 ,  C12Q1/6853 ,  C12Q1/686 ,  B01J2219/00317 ,  B01J2219/00454 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00554 ,  B01J2219/00576 ,  B01J2219/00619 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0864 ,  G01N2035/00158 ,  C12Q1/6853 ,  C12Q2563/149 ,  C12Q2565/629 ,  C12Q1/6823 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent/support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

公开(公告)号：US20240287592A1

公开(公告)日：2024-08-29

申请号：US18562953

申请日：2022-06-09

Applicant: New England Biolabs, Inc.

Inventor： Nathan Tanner ,  Ryan T. Fuchs ,  Jennifer L. Curcuru ,  G. Brett Robb

IPC: C12Q1/6844 ,  C12N9/12 ,  C12N9/22 ,  C12Q1/48 ,  C12Q1/6823

CPC classification number: C12Q1/6844 ,  C12N9/1252 ,  C12N9/22 ,  C12Q1/485 ,  C12Q1/6823 ,  C12Y207/07007 ,  C12Y207/07049

Abstract: Kits and methods are provided that utilize a mesophilic strand displacing polymerase selected from Bsu DNA polymerase (large fragment) and Klenow in Loop mediated amplification (LAMP) at temperatures in the range of 34° C.-52ºC. This contrasts with 60° C.-65° C. required for standard Bst polymerase dependent LAMP. The reduced temperature of the LAMP reaction enables the use of other proteins that are temperature sensitive in a one-step reaction. For example, a Cas protein such as Cas12a may be used with a target nucleic acid specific guide RNA and optionally a reporter oligonucleotide containing a quencher and a fluorophore or lateral flow reagents to determine the presence of pathogens in a sample.

公开(公告)号：US12071652B2

公开(公告)日：2024-08-27

申请号：US17323695

申请日：2021-05-18

Applicant: Purdue Research Foundation

Inventor： Casey J. Krusemark ,  Kyle Denton ,  Dongwook Kim ,  Rachael Jetson

IPC: C12Q1/6823 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/686 ,  G01N33/68

CPC classification number: C12Q1/6823 ,  C07H21/04 ,  C12N15/1065 ,  C12N15/1075 ,  C12Q1/686 ,  G01N33/68 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N15/1075 ,  C12Q2521/537 ,  C12Q2563/179 ,  C12Q1/6823 ,  C12Q2521/537 ,  C12Q2563/179

Abstract: Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.

公开(公告)号：US20240271206A1

公开(公告)日：2024-08-15

申请号：US18392547

申请日：2023-12-21

Applicant: Illumina, Inc.

Inventor： Antoine Francais ,  Elena Cressina ,  Kathryn Hattingh ,  Angelica Mariani ,  Xiaolin Wu ,  Xiaohai Liu

IPC: C12Q1/6874 ,  B01J23/44 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6823

CPC classification number: C12Q1/6874 ,  B01J23/44 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6823 ,  G01N2333/9126

Abstract: Embodiments of the present disclosure relate to nucleotides with 3′ allyl blocking groups. Also provided herein are methods of sequencing using nucleotides with 3′ allyl blocking groups described herein, and sequencing kits.

公开(公告)号：US20240263216A1

公开(公告)日：2024-08-08

申请号：US18410793

申请日：2024-01-11

Applicant: Ultima Genomics, Inc.

Inventor： Linda G. LEE ,  Gilad ALMOGY ,  Steven MENCHEN

IPC: C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: The present disclosure provides labeling reagents for labeling substrates such as nucleotides, proteins, antibodies, lipids, and cells. The labeling reagents provided herein may comprise fluorescent labels and semi-rigid linkers. Methods for nucleic acid sequencing using materials comprising such labeling reagents are also provided here.