公开(公告)号：US20080166769A1

公开(公告)日：2008-07-10

申请号：US11921329

申请日：2006-06-01

申请人： Tadayuki Uno ,  Yohei Hoashi ,  Naohisa Katayama ,  Toshiya Kai

发明人： Tadayuki Uno ,  Yohei Hoashi ,  Naohisa Katayama ,  Toshiya Kai

IPC分类号： C12N15/63 ,  C12N1/21

CPC分类号： C12N15/70 ,  C07K14/805 ,  C12N15/69 ,  C12P21/02

摘要： Provided is a high-copy-number, high-expression vector capable of producing a protein having satisfactory functions and activity of the same level as that of a natural form of the protein, in a large quantity and in a simple manner. Also provided is a vector including: (A) a target gene or a cloning site of the target gene, (B) a sequence element necessary for the high copying of the target gene, (C) a sequence element necessary for the expression of the target gene and a methionine aminopeptidase gene; a method of producing the vector; a transformant having the vector introduced therein; and a process for producing a protein using the transformant.

摘要翻译： 提供了能够以大量和简单的方式产生具有与天然形式的蛋白质相同水平的令人满意的功能和活性的蛋白质的高拷贝数高表达载体。 还提供了载体，其包括：（A）靶基因的靶基因或克隆位点，（B）靶基因的高拷贝所必需的序列元件，（C）表达所需靶序列所需的序列元件 靶基因和甲硫氨酸氨基肽酶基因; 生成载体的方法; 其中引入载体的转化体; 以及使用该转化体生产蛋白质的方法。