公开(公告)号：US5789152A

公开(公告)日：1998-08-04

申请号：US209261

申请日：1994-03-16

申请人： Christopher Black ,  Pierre-Francios Tosi ,  Andrew Atkin ,  Jaime E. Lazarte ,  Yves Claude Nicolau

发明人： Christopher Black ,  Pierre-Francios Tosi ,  Andrew Atkin ,  Jaime E. Lazarte ,  Yves Claude Nicolau

IPC分类号： G01N33/543 ,  G01N33/544 ,  G01N33/555 ,  G01N33/569 ,  C12Q1/70

CPC分类号： B82Y5/00 ,  G01N33/5432 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974 ,  Y10S436/829

摘要： The present invention encompasses membranous baculovirus-derived vesicle compositions having exposed on their external surfaces a ligator capable of selectively binding a ligand to be detected. The compositions also have exposed on their outer surfaces a signal reagent capable of reacting with another signal reagent to generate a signal capable of being detected. The present invention also encompasses assay methods for determining the presence and amount of a ligand in a sample using the membranous vesicle compositions of the present invention.

摘要翻译： 本发明包括膜外杆状病毒衍生的囊泡组合物，其在其外表面上暴露出能够选择性结合要检测的配体的连接子。 组合物还在其外表面上暴露出能够与另一个信号试剂反应以产生能够被检测的信号的信号试剂。 本发明还包括使用本发明的膜泡囊组合物来测定样品中配体的存在和量的测定方法。