公开(公告)号：US08945866B2

公开(公告)日：2015-02-03

申请号：US11837006

申请日：2007-08-10

申请人： Stefan Niehren

发明人： Stefan Niehren

IPC分类号： G01N1/30 ,  G01N33/48 ,  G01N1/00 ,  G01N1/28 ,  G02B21/32

CPC分类号： G01N1/2813 ,  G01N2001/282 ,  G01N2001/284 ,  G02B21/32

摘要： Methods and devices for cutting and collecting dissected specimens are described herein. In one embodiment, the method for dissecting and collecting specimens includes the following steps: placing a sample onto a slide of a laser dissection microscope, the slide being translatable in its plane of extension, lowering an adherent collection device centered on the optical axis onto the probe, wherein the adherent collection device, in its lowered state, adheres to the sample, is freely translatable with the slide and decoupled from the optical axis of the microscope, and, in its raised state, is fixed relative to the optical axis, dissecting one or more specimens from the sample, wherein the following steps are performed if the next specimen to be dissected is located outside of a predetermined collection radius of the adherent collection device: raising the adherent collection device, translating the slide to a given position relative to the optical axis of the microscope in order to decenter the center of the specimen to be dissected from the optical axis of the microscope, lowering the adherent collection device onto the sample, and dissecting one or more subsequent specimens, wherein, after each step of raising the adherent collection device, the predetermined position is spaced apart from all previously determined positions by at least one predetermined distance.

摘要翻译： 本文描述了用于切割和收集解剖标本的方法和装置。 在一个实施例中，用于解剖和收集标本的方法包括以下步骤：将样品放置在激光解剖显微镜的载玻片上，所述载玻片可在其延伸平面中平移，将以光轴为中心的粘附收集装置降低到 探针，其中处于其降低状态的粘附收集装置粘附到样品，可随着载玻片自由平移并与显微镜的光轴分离，并且在其升高状态下相对于光轴固定，解剖 一个或多个来自样品的样品，其中如果要解剖的下一个样品位于粘附收集装置的预定收集半径之外，则执行以下步骤：升高粘附收集装置，将载玻片相对于相对于 显微镜的光轴为了将要从显微镜的光轴上解剖的样本的中心倾斜， 将粘附的收集装置降低到样品上，并且解剖一个或多个随后的样品，其中在提高粘附收集装置的每个步骤之后，预定位置与所有先前确定的位置间隔至少一个预定距离。

公开(公告)号：US08535905B2

公开(公告)日：2013-09-17

申请号：US10202118

申请日：2002-07-24

申请人： Norbert Leclerc

发明人： Norbert Leclerc

IPC分类号： G01N33/48

CPC分类号： G01N1/2813 ,  G01N2001/2833

摘要： A film (14) with a tissue section (10) is placed with the tissue section (10) downward on a microscope slide (18) and the microscope slide (18) positioned in the object plane of an inverse microscope; where an adhesive tape (20) is arranged, with a bonding agent (22) downward, above the film (14) and therefore above the tissue section (10); wherein the next step, tissue (36) to be isolated is excised by a focused laser beam, which also divides the film (14); whereupon removal of the adhesive tape (20) from the microscope, the excised tissue pieces (36) adhere to the adhesive tape (20), and the remnant of the film (14) and of the tissue section (10) remains adhering/to the microscope slide (18).