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101.发明申请Plants having improved growth characteristics and method for making the same 审中-公开具有改善生长特性的植物及其制备方法公开(公告)号：US20090271895A1
公开(公告)日：2009-10-29
申请号：US11632580
申请日：2005-07-14
申请人： Valerie Frankard , Vladimir Mironov
发明人： Valerie Frankard , Vladimir Mironov
CPC分类号： C12N15/8262 , C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： The present invention concerns a method for improving growth characteristics of plants by increasing activity and/or expression in a plant of an SnRK2 kinase or a homologue thereof. One such method comprises introducing into a plant an SnRK2 nucleic acid molecule or functional variant thereof. The invention also relates to transgenic plants having improved growth characteristics, which plants have modulated expression of a nucleic acid encoding an SnRK2 kinase. The present invention also concerns constructs useful in the methods of the invention.
摘要翻译： 本发明涉及通过增加SnRK2激酶或其同源物的植物中的活性和/或表达来改善植物生长特性的方法。 一种这样的方法包括向植物中引入SnRK2核酸分子或其功能变体。 本发明还涉及具有改善的生长特性的转基因植物，该植物已经调节了编码SnRK2激酶的核酸的表达。 本发明还涉及可用于本发明方法的构建体。
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公开(公告)号：US20080229445A1
公开(公告)日：2008-09-18
申请号：US10584024
申请日：2004-12-22
申请人： Valerie Frankard , Vladmir Mironov
发明人： Valerie Frankard , Vladmir Mironov
CPC分类号： C12N15/8261 , Y02A40/146
摘要： The invention concerns a method for increasing plant yield by introducing into a plant a cyclin A nucleic acid, preferably encoding a cyclin A protein, which cyclin A nucleic acid is operably linked to a seed-preferred promoter. By using this method, plant yield may be increased in optimal and sub-optimal growing conditions. The method results in plants having increased yield relative to corresponding wild type plants and relative to transgenic plants constitutively expressing cyclin A.
摘要翻译： 本发明涉及通过将植物细胞周期蛋白A核酸（优选编码细胞周期蛋白A蛋白）引入植物来提高植物产量的方法，该细胞周期蛋白A核酸可操作地连接到种子优选的启动子。 通过使用该方法，可以在优化和次优生长条件下提高植物产量。 该方法导致植物相对于相应的野生型植物和相对于组成型表达细胞周期蛋白A的转基因植物具有增加的产量。
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103.发明申请公开(公告)号：US20070199085A1
公开(公告)日：2007-08-23
申请号：US10570554
申请日：2004-09-03
CPC分类号： C12N9/1205 , C12N15/8262 , C12Q1/485 , G01N2333/39 , G01N2500/10
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
摘要翻译： 本发明涉及通过调节B型CDK核酸的植物表达和/或调节B型CDK蛋白的植物中的活性和/或水平来修饰植物生长特性的方法。 本发明还涉及具有修饰的生长特性的转基因植物，相对于表达，活性，植物调节B型CDK核酸的表达和/或调节的活性和/或B型CDK蛋白的植物中的水平 /或相应野生型植物中的水平。 本发明还提供了一种用于鉴定相对于相应的非突变CDKs具有增强的CDK活性的突变CDK的新型筛选方法。 本发明还提供了用于鉴定能够结合CKI（细胞周期蛋白依赖性激酶抑制剂）的非活性CDK的新型筛选方法。 本发明还提供可通过本发明的筛选方法获得的突变体CDK。
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公开(公告)号：US20050223429A1
公开(公告)日：2005-10-06
申请号：US11057473
申请日：2005-02-14
CPC分类号： C12N9/0032 , C12N15/8229 , C12N15/823 , C12N15/8261 , C12N15/827 , Y02A40/146
摘要： The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
摘要翻译： 本发明涉及用于刺激根生长和/或增强侧根或不定根形成和/或改变根向地力的方法，包括细胞分裂素氧化酶的表达或包括降低植物或植物中活性细胞分裂素水平的另一种蛋白质的表达 部分。 本发明还提供了增加种子大小和/或重量，胚胎大小和/或重量以及子叶大小和/或重量的方法。 该方法包括细胞分裂素氧化酶的表达或另一种降低植物或植物部分活性细胞分裂素水平的蛋白质的表达。 还提供了用于提高种子产量的方法和组合物。 本发明还涉及分离的植物细胞分裂素氧化酶蛋白，编码细胞分裂素氧化酶蛋白的核酸序列以及包含这些序列的载体，宿主细胞，转基因细胞和植物。 这些序列用于改善根相关特征，包括增加产量和/或增强早期活力和/或改善根/芽比，和/或改善抗倒伏和/或增加耐旱性和/或促进外植体的体外繁殖 和/或修饰细胞命运和/或植物发育和/或植物形态和/或植物生物化学和/或植物生理学。 本发明还涉及用于鉴定和获得与细胞分裂素氧化酶蛋白相互作用的蛋白质和化合物以及使用这些蛋白质和/或化合物作为植物生长调节剂或除草剂的方法。
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105.发明申请Method to modify cell number, architecture and yield of plants by overexpressing the e2f transcription factor 失效通过过表达e2f转录因子来修饰植物的细胞数量，结构和产量的方法公开(公告)号：US20050059154A1
公开(公告)日：2005-03-17
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译： 本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。 更具体地，本发明涉及刺激植物细胞分裂的方法。 本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。 优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。
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