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1.发明授权Method to modify cell number, architecture and yield of plants by overexpressing the E2F transcription factor 失效通过过表达E2F转录因子修饰植物的细胞数量，结构和产量的方法公开(公告)号：US07592507B2
公开(公告)日：2009-09-22
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译： 本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。 更具体地，本发明涉及刺激植物细胞分裂的方法。 本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。 优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。
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2.发明申请Method to modify cell number, architecture and yield of plants by overexpressing the e2f transcription factor 失效通过过表达e2f转录因子来修饰植物的细胞数量，结构和产量的方法公开(公告)号：US20050059154A1
公开(公告)日：2005-03-17
申请号：US10489500
申请日：2002-09-12
申请人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
发明人： Tom Beeckman , Lieven De Veylder , Dirk Inze , Vladimir Mironov , Willem Broekaert , Willy Dillen , Valerie Frankard
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译： 本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。 更具体地，本发明涉及刺激植物细胞分裂的方法。 本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。 优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。
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3.发明申请Plants Having Improved Growth Characteristics And A Method For Making The Same 审中-公开具有改善生长特性的植物及其制作方法公开(公告)号：US20090222952A1
公开(公告)日：2009-09-03
申请号：US12092844
申请日：2006-11-08
申请人： Jan de Meutter , Vladimir Mironov , Christophe Reuzeau , Dirk Inze , Kobe Vlieghe , Lieven de Veylder
发明人： Jan de Meutter , Vladimir Mironov , Christophe Reuzeau , Dirk Inze , Kobe Vlieghe , Lieven de Veylder
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63 , C07K14/415 , C12Q1/68
CPC分类号： C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention concerns a method for increasing plant yield and/or increased stress resistance by decreasing the level of activity of a DEL1 polypeptide. One such method comprises introducing into a plant a nucleic acid comprising a variant DEL1 nucleic acid. Another method comprises downregulating expression of a DEL1 gene. The invention also relates to transgenic plants having introduced therein a variant DEL1 nucleic acid thereof, which plants have increased yield and/or increased stress resistance relative to control plants. The present invention also concerns a novel DEL1 protein and its encoding sequence, and constructs useful in the methods of the invention.
摘要翻译： 本发明涉及通过降低DEL1多肽的活性水平来提高植物产量和/或增加的胁迫抗性的方法。 一种这样的方法包括向植物中引入包含变体DEL1核酸的核酸。 另一种方法包括下调DEL1基因的表达。 本发明还涉及其中引入了其变体DEL1核酸的转基因植物，该植物相对于对照植物具有增加的产量和/或增加的胁迫抗性。 本发明还涉及一种新颖的DEL1蛋白及其编码序列，以及可用于本发明方法的构建体。
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4.发明授权Plants having improved growth characteristics and a method for making the same 有权具有改善的生长特性的植物及其制备方法公开(公告)号：US08853492B2
公开(公告)日：2014-10-07
申请号：US11799083
申请日：2007-04-30
申请人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
发明人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
CPC分类号： C07K14/415 , C12N9/1205 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译： 本发明一般涉及分子生物学领域，涉及相对于相应野生型植物改善植物生长特性的方法。 更具体地，本发明涉及一种改善植物生长特性的方法，包括调节植物中编码I型同源域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的表达; 或包括调节植物中编码硝酸转运蛋白（NRT）或其同源物的核酸的表达; 或包括调节植物中编码称为产量增强蛋白16（YEP16）的多肽的核酸的表达; 或包括调节I型糖原合酶激酶（I类绒毛样激酶）植物中的表达或其同源物。 本发明还涉及具有调节I型HDZip hox5多肽或其同源物的核酸表达的植物; 或已调节编码NRT蛋白或其同源物的核酸的表达; 或已调节编码表示为YEP16的多肽的核酸的表达; 或具有调节I组粗毛状激酶或其同源物的表达，该植物相对于相应的野生型植物具有改善的生长特性。 本发明还提供了可用于本发明方法的构建体。
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5.发明申请Plants having improved growth characteristics and a method for making the same 有权具有改善的生长特性的植物及其制备方法公开(公告)号：US20080127365A1
公开(公告)日：2008-05-29
申请号：US11799083
申请日：2007-04-30
申请人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
发明人： Ana Isabel Sanz Molinero , Christophe Reuzeau , Yves Hatzfeld , Veronique Boudolf , Lieven de Veylder , Dirk Inze , Vladimir Mironov
CPC分类号： C07K14/415 , C12N9/1205 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译： 本发明一般涉及分子生物学领域，涉及相对于相应野生型植物改善植物生长特性的方法。 更具体地，本发明涉及一种改善植物生长特性的方法，包括调节植物中编码I型同源域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的表达; 或包括调节植物中编码硝酸转运蛋白（NRT）或其同源物的核酸的表达; 或包括调节植物中编码称为产量增强蛋白16（YEP16）的多肽的核酸的表达; 或包括调节I型糖原合酶激酶（I类绒毛样激酶）植物中的表达或其同源物。 本发明还涉及具有调节I型HDZip hox5多肽或其同源物的核酸表达的植物; 或已调节编码NRT蛋白或其同源物的核酸的表达; 或已调节编码表示为YEP16的多肽的核酸的表达; 或具有调节I组粗毛状激酶或其同源物的表达，该植物相对于相应的野生型植物具有改善的生长特性。 本发明还提供了可用于本发明方法的构建体。
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6.发明授权Method and means for modulating plant cell cycle proteins and their use in plant cell growth control 有权调节植物细胞周期蛋白的方法和手段及其在植物细胞生长控制中的应用公开(公告)号：US07825295B2
公开(公告)日：2010-11-02
申请号：US11698644
申请日：2007-01-26
申请人： Dirk Inze , Gerda Segers , Lieven De Veylder , Vladimir Mironov
发明人： Dirk Inze , Gerda Segers , Lieven De Veylder , Vladimir Mironov
IPC分类号： C12N15/82
CPC分类号： C07K14/315 , C07K14/415 , C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a method for modulating endoreduplication in a plant, plant part, or plant cell. The method comprises the steps of introduction and expression of a nucleotide sequence encoding the CDC2bAt cyclin dependent kinase gene disclosed in database accession number X57840 or said CDC2bAt cyclin dependent kinase gene having the Asp residue at position 161 replaced with an Asn residue, under the control of a promoter which functions in a plant.
摘要翻译： 本发明提供了一种用于调节植物，植物部分或植物细胞中的重复序列的方法。 该方法包括以下步骤：在数据库登录号X57840中公开的编码CDC2bAt细胞周期依赖性激酶基因的核苷酸序列或所述CDC2bAt细胞周期依赖性激酶基因的引入和表达，所述CDC2bAt细胞周期蛋白依赖性激酶基因在位置161处的Asp残基被Asn残基取代， 在植物中起作用的启动子。
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7.发明授权Method and means for modulating plant cell cycle proteins and their use in plant cell growth control 有权调节植物细胞周期蛋白的方法和手段及其在植物细胞生长控制中的应用公开(公告)号：US06465718B1
公开(公告)日：2002-10-15
申请号：US09381150
申请日：2000-03-13
申请人： Dirk Inze , Gerda Segers , Lieven De Veylder , Vladimir Mironov
发明人： Dirk Inze , Gerda Segers , Lieven De Veylder , Vladimir Mironov
IPC分类号： C12N510
CPC分类号： C07K14/315 , C07K14/415 , C12N9/1205 , C12N15/8261 , Y02A40/146
摘要： The present invention provides a new Arabidopsis thaliana nucleotide sequence and polypeptide sequence having a molecular weight of about 10.5 kDa. Modulation of the expression of the polypeptides encoded by the nucleotide sequences according to the invention has an advantageous influence on plant cell division characteristics especially on the endoreduplication whereby plant cell size and storage capacity of plant cells is influenced.
摘要翻译： 本发明提供了具有约10.5kDa分子量的新拟南芥核苷酸序列和多肽序列。 由根据本发明的核苷酸序列编码的多肽的表达的调节对植物细胞分裂特征具有有利影响，特别是对植物细胞的植物细胞大小和储存能力的影响。
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8.发明申请公开(公告)号：US20070199085A1
公开(公告)日：2007-08-23
申请号：US10570554
申请日：2004-09-03
CPC分类号： C12N9/1205 , C12N15/8262 , C12Q1/485 , G01N2333/39 , G01N2500/10
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
摘要翻译： 本发明涉及通过调节B型CDK核酸的植物表达和/或调节B型CDK蛋白的植物中的活性和/或水平来修饰植物生长特性的方法。 本发明还涉及具有修饰的生长特性的转基因植物，相对于表达，活性，植物调节B型CDK核酸的表达和/或调节的活性和/或B型CDK蛋白的植物中的水平 /或相应野生型植物中的水平。 本发明还提供了一种用于鉴定相对于相应的非突变CDKs具有增强的CDK活性的突变CDK的新型筛选方法。 本发明还提供了用于鉴定能够结合CKI（细胞周期蛋白依赖性激酶抑制剂）的非活性CDK的新型筛选方法。 本发明还提供可通过本发明的筛选方法获得的突变体CDK。
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公开(公告)号：US20080163394A1
公开(公告)日：2008-07-03
申请号：US11887056
申请日：2006-03-24
申请人： Valerie Frankard , Vladimir Mironov
发明人： Valerie Frankard , Vladimir Mironov
CPC分类号： C12N15/8261 , Y02A40/146
摘要： The present invention concerns a method for increasing plant yield relative to corresponding wild type plants. More specifically, the present invention concerns a method for increasing plant yield comprising introducing into a plant a nucleic acid encoding a cyclin D3 (CYCD3) polypeptide under the control of a promoter capable of preferentially expressing the nucleic acid in the endosperm of seeds. The present invention also concerns plants comprising an isolated nucleic acid encoding a CYCD3 polypeptide under the control of a promoter capable of preferentially expressing the nucleic acid in the endosperm of seeds, which plants have increased yield relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译： 本发明涉及相对于相应野生型植物提高植物产量的方法。 更具体地说，本发明涉及一种用于增加植物产量的方法，包括在能够优先在种子的胚乳中表达核酸的启动子的控制下向植物中引入编码细胞周期蛋白D3（CYCD3）多肽的核酸。 本发明还涉及包含编码CYCD3多肽的分离的核酸的植物，所述核酸在能够优先在种子的胚乳中表达核酸的启动子控制下，该植物相对于相应的野生型植物具有增加的产量。 本发明还提供了可用于本发明方法的构建体。
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10.发明申请公开(公告)号：US20070118931A1
公开(公告)日：2007-05-24
申请号：US10583212
申请日：2004-12-17
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a nucleic acid sequence encoding a GRUBX protein and/or modulating activity and/or levels in a plant of a GRUBX protein. The present invention furthermore provides novel GRUBX proteins and nucleic acids encoding such proteins. The invention also relates to constructs comprising GRUBX encoding nucleic acids, and transgenic plants having modified growth characteristics, which plants have modulated expression of a nucleic acid encoding a GRUBX protein.
摘要翻译： 本发明涉及一种通过调节植物中编码GRUBX蛋白的核酸序列表达和/或调节GRUBX蛋白植物中的活性和/或水平来修饰植物生长特性的方法。 本发明还提供了编码这种蛋白质的新型GRUBX蛋白质和核酸。 本发明还涉及包含GRUBX编码核酸的构建体和具有修饰生长特性的转基因植物，该植物已经调节了编码GRUBX蛋白质的核酸的表达。
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