公开(公告)号：US08921086B2

公开(公告)日：2014-12-30

申请号：US11645223

申请日：2006-12-21

Applicant: David K. Hanzel ,  Geoff Otto ,  Paul Peluso ,  Thang Pham ,  David R. Rank ,  Paul Mitsis ,  Fred Christians ,  Arek Bibillo ,  Insil Park ,  Sonya Clark ,  John Lyle

Inventor： David K. Hanzel ,  Geoff Otto ,  Paul Peluso ,  Thang Pham ,  David R. Rank ,  Paul Mitsis ,  Fred Christians ,  Arek Bibillo ,  Insil Park ,  Sonya Clark ,  John Lyle

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C07K2319/20 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Y207/07007 ,  Y02P20/52

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract translation: 提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。 还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。