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公开(公告)号：US07455978B2
公开(公告)日：2008-11-25
申请号：US10748720
申请日：2003-12-30
CPC分类号： G01N33/502 , C07K1/113
摘要： Many proteins, when produced recombinantly, suffer from improper processing, folding and lack normal solubility. Modified proteins, including those indicative of disease states, also can have such defects. The present invention is directed to methods of identifying proper and improper protein folding, aberrant processing and/or insolubility. The method relies on the use of two components: a specialized fusion protein and structural complementation. The fusion protein contains sequences from the protein of interest and one portion of a marker protein that, by itself, is not active. A host cell then provides the remainder of the marker protein that serves to “complement” the function of the fused marker protein such that their association restores activity, permitting detection.
摘要翻译： 当重组产生时，许多蛋白质经受不当的加工，折叠和缺乏正常的溶解度。 修饰的蛋白质，包括指示疾病状态的蛋白质，也可能具有这样的缺陷。 本发明涉及鉴定正确和不适当的蛋白质折叠，异常加工和/或不溶性的方法。 该方法依赖于两个组成部分的使用：一种专门的融合蛋白和结构互补。 融合蛋白包含来自目的蛋白质的序列和一部分本身不具有活性的标记蛋白质。 宿主细胞然后提供标记蛋白的其余部分，其用于“补充”融合标记蛋白的功能，使得它们的缔合恢复活性，允许检测。
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公开(公告)号：US06727070B2
公开(公告)日：2004-04-27
申请号：US09775051
申请日：2001-01-31
IPC分类号： G01N3353
CPC分类号： G01N33/502 , C07K1/113
摘要： Many proteins, when produced recombinantly, suffer from improper processing, folding and lack normal solubility. Modified proteins, including those indicative of disease states, also can have such defects. The present invention is directed to methods of identifying proper and improper protein folding, aberrant processing and/or insolubility. The method relies on the use of two components: a specialized fusion protein and structural complementation. The fusion protein contains sequences from the protein of interest and one portion of a marker protein that, by itself, is not active. A host cell then provides the remainder of the marker protein that serves to “complement” the function of the fused marker protein such that their association restores activity, permitting detection.
摘要翻译： 当重组产生时，许多蛋白质经受不当的加工，折叠和缺乏正常的溶解度。 修饰的蛋白质，包括指示疾病状态的蛋白质，也可能具有这样的缺陷。 本发明涉及鉴定正确和不适当的蛋白质折叠，异常加工和/或不溶性的方法。 该方法依赖于两个组成部分的使用：一种专门的融合蛋白和结构互补。 融合蛋白包含来自目的蛋白质的序列和一部分本身不具有活性的标记蛋白质。 宿主细胞然后提供标记蛋白的其余部分，其用于“补充”融合标记蛋白的功能，使得它们的缔合恢复活性，允许检测。
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13.发明授权Method and apparatus for forming three dimensional structural components from wood fiber 失效从木纤维形成三维结构部件的方法和装置
公开(公告)号：US4702870A
公开(公告)日：1987-10-27
申请号：US864920
申请日：1986-05-20
申请人： Vance C. Setterholm , John F. Hunt
发明人： Vance C. Setterholm , John F. Hunt
CPC分类号： B28B1/525 , B27N5/00 , B28B3/003 , B28B3/024 , B28B7/348 , Y10S425/019 , Y10S425/044 , Y10S425/112 , Y10S425/119 , Y10T428/24149
摘要： Methods and apparatus for producing three-dimensional structural members having one flat side from wood fibers utilizing flow deposition of the fibers onto a support. The support includes resilient deformable mold inserts. The mold inserts are formed of silicone rubber and may be blocks of the material, or may be a membrane which can be inflated to form the mold inserts. Pressing is done with a force normal to the support, and the mold inserts create response forces at varying directions to thus shape and hold the three-dimensional finished part. Heat drying also may be used.
摘要翻译： 用于生产具有木纤维的一个平坦侧面的三维结构构件的方法和装置，其利用将纤维流动沉积到载体上。 支撑件包括弹性可变形模具插入件。 模具插入件由硅橡胶形成，并且可以是该材料的块体，或者可以是可膨胀以形成模具插入件的膜。 用垂直于支撑件的力进行压制，并且模具插件在变化的方向上产生响应力，从而形成并保持三维成品部分。 也可以使用热干燥。
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公开(公告)号：US4381023A
公开(公告)日：1983-04-26
申请号：US238401
申请日：1981-02-26
申请人： Frank J. Fronczak , John F. Hunt
发明人： Frank J. Fronczak , John F. Hunt
摘要： A power back-up roll for a rotary veneer lathe comprises a drive roller for applying a rotational force to the outer periphery of a log axially mounted on the veneer lathe, a controllable drive source for driving the roller and a servo-control system for maintaining the static friction between the roller and the log. In a preferred embodiment of the invention, the servocontrol system includes a first DC-generator tachometer for monitoring the peripheral speed of the log, a second DC-generator tachometer for monitoring the peripheral speed of the roller; and a feedback loop, operatively associated with the controllable drive source as well as the first and second tachometers, for matching the peripheral speed of the roller to that of the log.
摘要翻译： 用于旋转单板车床的动力支撑辊包括用于向轴向安装在单板车床上的原木的外周施加旋转力的驱动辊，用于驱动辊的可控驱动源和用于维持的伺服控制系统 辊与原木之间的静摩擦力。 在本发明的优选实施例中，伺服控制系统包括用于监测原木的圆周速度的第一DC发生器转速计，用于监测辊的圆周速度的第二DC发生器转速计; 以及与可控驱动源以及第一和第二转速计可操作地相关联的反馈回路，用于将辊的圆周速度与日志的圆周速度相匹配。
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公开(公告)号：US20130199743A1
公开(公告)日：2013-08-08
申请号：US13757603
申请日：2013-02-01
申请人： Christine M. Lee , John F. Hunt
发明人： Christine M. Lee , John F. Hunt
IPC分类号： D21J1/04
CPC分类号： D21J1/04
摘要： The present invention relates to pressed fiberboard articles made using a binderless wet process from a combination of fibers and particles. A mixture of particles (e.g., wood particles) and cellulosic fibers is formed into a panel using only water, heat and pressure. The present invention is further directed to fiberboard articles having uniform density cores or higher density cores.
摘要翻译： 本发明涉及使用无粘合剂湿法从纤维和颗粒的组合制成的压制纤维板制品。 仅使用水，热和压力将颗粒（例如木颗粒）和纤维素纤维的混合物形成为面板。 本发明还涉及具有均匀密度核心或更高密度核心的纤维板制品。
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公开(公告)号：US07635569B2
公开(公告)日：2009-12-22
申请号：US11617191
申请日：2006-12-28
CPC分类号： G01N33/502 , C07K1/113
摘要： Many proteins, when produced recombinantly, suffer from improper processing, folding and lack normal solubility. Modified proteins, including those indicative of disease states, also can have such defects. The present invention is directed to methods of identifying proper and improper protein folding, aberrant processing and/or insolubility. The method relies on the use of two components: a specialized fusion protein and structural complementation. The fusion protein contains sequences from the protein of interest and one portion of a marker protein that, by itself, is not active. A host cell then provides the remainder of the marker protein that serves to “complement” the function of the fused marker protein such that their association restores activity, permitting detection.
摘要翻译： 当重组产生时，许多蛋白质经受不当的加工，折叠和缺乏正常的溶解度。 修饰的蛋白质，包括指示疾病状态的蛋白质，也可能具有这样的缺陷。 本发明涉及鉴定正确和不适当的蛋白质折叠，异常加工和/或不溶性的方法。 该方法依赖于两个组成部分的使用：一种专门的融合蛋白和结构互补。 融合蛋白包含来自目的蛋白质的序列和一部分本身不具有活性的标记蛋白质。 宿主细胞然后提供标记蛋白的其余部分，其用于“补充”融合标记蛋白的功能，使得它们的缔合恢复活性，允许检测。
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公开(公告)号：US20080214947A1
公开(公告)日：2008-09-04
申请号：US11660653
申请日：2005-08-19
申请人： John F. Hunt , Brian K. Walsh , Alfred R. Baddour , Dan Mackey
发明人： John F. Hunt , Brian K. Walsh , Alfred R. Baddour , Dan Mackey
IPC分类号： A61B5/00
CPC分类号： A61B5/14539 , A61B5/083 , A61B5/097
摘要： A method and system that provides for minute-to-minute EBC pH monitoring (or for other EBC characteristic monitoring) of a subject that greatly assists in determining the time-course of airway pH changes (or other characteristic changes) in evolving disease processes, and may assist in determining the predictive ability of these tests, as well as determining response to therapy.
摘要翻译： 提供一个方法和系统，提供一个主题的分钟至EBC pH监测（或其他EBC特征监测），这大大有助于确定进化疾病过程中气道pH变化（或其他特征变化）的时间过程， 并且可以帮助确定这些测试的预测能力，以及确定对治疗的反应。
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公开(公告)号：US5739273A
公开(公告)日：1998-04-14
申请号：US303651
申请日：1994-09-09
申请人： Donald M. Engelman , John F. Hunt
发明人： Donald M. Engelman , John F. Hunt
IPC分类号： C07K14/215 , A61K38/00 , A61K38/02 , C07K5/00 , C07K7/00
CPC分类号： C07K14/215
摘要： A water-soluble polypeptide having the characteristics of the bacteriorhodopsin C helix such that it is largely hydrophobic and spontaneously inserts into a biological membrane as an alpha-helix. The polypeptide is administered as an aqueous solution or is contained within the lipid bilayer of a liposome. The polypeptide is modified so that it will interfere with the helix--helix interactions within the membranes of adverse cells or is coupled to a therapeutic substance, such as a protein or pharmaceutical compound, for transmission thereof through the membrane and into the cell.
摘要翻译： 具有细菌视紫红质C螺旋的特征的水溶性多肽，使得其大部分是疏水性的并且自发地插入生物膜作为α-螺旋。 多肽作为水溶液施用或者包含在脂质体的脂质双层内。 修饰多肽使得它将干扰不良细胞的膜内的螺旋 - 螺旋相互作用，或者与治疗物质例如蛋白质或药物化合物偶联，以将其透过膜并进入细胞。
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公开(公告)号：US4737056A
公开(公告)日：1988-04-12
申请号：US923878
申请日：1986-10-28
申请人： John F. Hunt
发明人： John F. Hunt
CPC分类号： B60P7/15
摘要： A brace for securing cargo inside a containing device, such as the cargo bed of a truck, includes a first cylindrical pole with mounting brackets at opposite ends of the pole for engaging the sidewalls of the container. The cargo brace is selectively adjustable along its lengthwise axis to decrease or increase its length. Furthermore, the mounting brackets are pivotally connected to the pole to permit the brace to be installed at a selected orientation to the sidewalls. In another embodiment, the aforementioned brace includes a T-connector which is mounted arond the first pole and further includes a second pole which is engaged to the T-connector and which is perpendicular to the first pole. The second pole includes a mounting bracket which engages another sidewall or floor of the container. In another embodiment, a sleeve, having flanged ends which are perpendicular to the sleeve, and a handle, is mounted about the first pole to secure the cargo in the container. Typically, pads fabricated of an appropriate elastomer are employed at the ends of the brace to prevent damage from metal-to-metal contact, and the base is surrounded with one or more sleeves of a polymeric material to prevent damage to cargo contacted by the brace.
摘要翻译： 用于将货物固定在容纳装置（例如卡车的货物）内的支架包括：第一圆柱形杆，其具有用于接合容器的侧壁的杆的相对端处的安装支架。 货物支架沿其纵向轴线可选择性地调节以减小或增加其长度。 此外，安装支架枢转地连接到杆，以允许支架以选定的方向安装到侧壁。 在另一个实施例中，前述的支架包括安装在第一极上的T形连接器，并且还包括与T形连接器接合且垂直于第一极的第二极。 第二杆包括与容器的另一个侧壁或地板接合的安装支架。 在另一个实施例中，具有垂直于套筒的凸缘端和手柄的套筒围绕第一极安装以将货物固定在容器中。 通常，在支架的端部使用由适当的弹性体制成的垫，以防止金属对金属接触的损坏，并且基座被一个或多个聚合物材料的套筒围绕，以防止由支架接触的货物的损坏 。
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公开(公告)号：US09740799B2
公开(公告)日：2017-08-22
申请号：US13991092
申请日：2011-12-02
IPC分类号： G06F17/50 , B29C53/06 , B29K105/08
CPC分类号： G06F17/50 , B29C53/063 , B29K2105/08
摘要： A three-dimensional engineered shaped fiber configuration is formed using determined structural requirements for a three-dimensional engineered shaped fiber configuration and ascertained properties of an engineered molded fiber fiberboard material. A first cut on a top surface and a second cut on a bottom surface of the fiberboard material are calculated. These calculations are based, at least in part, on the structural requirements properties of the fiberboard material. The first cut and the second cut each have a depth, a width, and a position. The first cut and the second cut have a spacing between them such that the flat piece of fiberboard material can be folded at a point located in the spacing between the first cut and second cut to position a first portion of the fiberboard material at a particular angle with respect to a second portion of the fiberboard material.
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