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11.发明授权Process for delaying the deactivation of glutaryl amidase during catalysis with a thiol 有权在硫醇催化过程中延迟戊二酰胺酶失活的方法公开(公告)号：US06395507B1
公开(公告)日：2002-05-28
申请号：US09578684
申请日：2000-05-26
IPC分类号： C12P3500
摘要： A process is provided for delaying the deactivation of glutaryl amidase during catalysis. The amidase is, after maximum conversion of a substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the amidase loses activity. The inactivation of the amidase can be delayed by bringing it into contact with a thiol during the reaction. The thiol is preferably 2-mercaptoethanol or cysteine.
摘要翻译： 提供了一种在催化过程中延迟戊二酰胺酶失活的方法。 酰胺酶是在底物最大转化后通过过滤分离出来的，可以在随后的反应批中重复使用。 在每个反应循环中，酰胺酶都会失去活性。 通过在反应期间使其与硫醇接触可以延迟酰胺酶的失活。 硫醇优选为2-巯基乙醇或半胱氨酸。
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公开(公告)号：US5426036A
公开(公告)日：1995-06-20
申请号：US30731
申请日：1993-03-12
CPC分类号： C07K14/62 , C07K14/36 , C12N15/76 , C07K2319/02 , C07K2319/75
摘要： The tendamistat gene can be used for the construction of fused genes with which fusion proteins are expressed and excreted in Streptomycetes host cells. The tendamistat portion can be modified, in particular it can be C-terminal shortened. When a gene for a shortened proinsulin in which the insulin B chain is linked to the A chain only via Lys or Lys-Lys is coupled to the tendamistat gene, this gene construction is introduced into an expression vector, and the latter is used to transform a Streptomycetes host cell, there is expression and secretion of the corresponding fusion protein. The fusion protein can easily be cleaved to give insulin precursors because of correctly established disulfide bonds. Genetic structures which code for the signal sequence and about the first ten amino acids of tendamistat as well as a desired protein are expressed in streptomyces host cells with a high yield, and the fusion proteins are secreted into the medium.
摘要翻译： 该趋化因子基因可用于构建融合蛋白的融合蛋白，其在链霉菌宿主细胞中表达和排泄。 可以修改卷曲部分，特别是可以缩短C端。 当胰岛素B链仅通过Lys或Lys-Lys与A链连接的缩短的胰岛素原的基因与趋化因子基因偶联时，将该基因构建引入表达载体中，并将后者用于转化 链霉菌宿主细胞，有相应融合蛋白的表达和分泌。 由于正确建立的二硫键，融合蛋白可以容易地被切割以产生胰岛素前体。 编码信号序列和约10个氨基酸的趋化因子以及所需蛋白质的遗传结构以高产量在链霉菌宿主细胞中表达，融合蛋白分泌到培养基中。
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公开(公告)号：US4770861A
公开(公告)日：1988-09-13
申请号：US13122
申请日：1987-02-10
CPC分类号： C01D17/00
摘要： Cesium chloride is recovered from used density gradient solutions by extraction of lipophilic and coloring components using a lipophilic solvent, subsequent extraction with a polar protic organic solvent, adding perchlorate ions to the extraction residue, and converting the precipitated cesium perchlorate into cesium chloride.
摘要翻译： 通过使用亲油溶剂提取亲油性和着色组分，然后用极性质子有机溶剂萃取，向萃取残余物中加入高氯酸根离子，并将沉淀的高氯酸铯转化成氯化铯，从已用密度梯度溶液中回收氯化铯。
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