公开(公告)号：US06649369B2

公开(公告)日：2003-11-18

申请号：US10118010

申请日：2002-04-09

申请人： Jürgen Bongs ,  Johannes Meiwes ,  Wolfgang Kruse ,  Klaus-Peter Koller

发明人： Jürgen Bongs ,  Johannes Meiwes ,  Wolfgang Kruse ,  Klaus-Peter Koller

IPC分类号： C12P3500

CPC分类号： C12N9/96 ,  C12N9/80

摘要： The present invention relates to a process for delaying the deactivation of glutaryl amidase during enzyme catalysis. The enzyme catalyst is, after maximum conversion of the substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the enzyme catalyst loses activity. The inactivation of the enzyme catalyst can be delayed by bringing it into contact with a thiol during the reaction.

公开(公告)号：US06395507B1

公开(公告)日：2002-05-28

申请号：US09578684

申请日：2000-05-26

申请人： Jürgen Bongs ,  Johannes Meiwes ,  Wolfgang Kruse ,  Klaus-Peter Koller

发明人： Jürgen Bongs ,  Johannes Meiwes ,  Wolfgang Kruse ,  Klaus-Peter Koller

IPC分类号： C12P3500

CPC分类号： C12N9/96 ,  C12N9/80

摘要： A process is provided for delaying the deactivation of glutaryl amidase during catalysis. The amidase is, after maximum conversion of a substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the amidase loses activity. The inactivation of the amidase can be delayed by bringing it into contact with a thiol during the reaction. The thiol is preferably 2-mercaptoethanol or cysteine.

摘要翻译： 提供了一种在催化过程中延迟戊二酰胺酶失活的方法。 酰胺酶是在底物最大转化后通过过滤分离出来的，可以在随后的反应批中重复使用。 在每个反应循环中，酰胺酶都会失去活性。 通过在反应期间使其与硫醇接触可以延迟酰胺酶的失活。 硫醇优选为2-巯基乙醇或半胱氨酸。

公开(公告)号：US5795750A

公开(公告)日：1998-08-18

申请号：US826881

申请日：1997-04-07

申请人： Wolfgang Kruse ,  Udo Kragl ,  Christian Wandrey

发明人： Wolfgang Kruse ,  Udo Kragl ,  Christian Wandrey

IPC分类号： C12M1/40 ,  C12P1/00 ,  C12P7/04 ,  C12P7/22 ,  C12P7/26 ,  C12P13/00 ,  C12M1/12 ,  C12N11/00 ,  C12P7/64

CPC分类号： C12P1/00 ,  C12M21/18 ,  C12M29/04 ,  C12M29/18 ,  C12P7/04 ,  C12P7/26 ,  Y02P20/582 ,  Y10S435/813 ,  Y10S435/817

摘要： In a process for the continuous enzyme-catalyticzed enzymatic production of hydrophobic products in an aqueous solution of a reaction mixture, the product is extracted from the product containing reaction mixture by way of a product-permeable microporous membrane into an organic solvent and the product-depleted reaction mixture is recycled.

摘要翻译： 在反应混合物的水溶液中连续酶催化生产疏水产物的方法中，通过产物渗透微孔膜将产物从含产物的反应混合物中提取到有机溶剂中， 贫化的反应混合物被回收。