公开(公告)号：US12116619B2

公开(公告)日：2024-10-15

申请号：US15640103

申请日：2017-06-30

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Sidi Chen ,  Randall Jeffrey Platt ,  Neville Espi Sanjana ,  Phillip A. Sharp ,  Feng Zhang

IPC分类号： C12Q1/6809 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12Q1/6886

CPC分类号： C12Q1/6809 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12Q1/6886 ,  C12N2310/20 ,  C12N2320/12 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q1/6809 ,  C12Q2521/301 ,  C12Q2525/207 ,  C12Q2535/122

摘要： The present invention relates to in vivo methods for modeling tumor formation and/or tumor evolution comprising the use of eukaryotic cells in which one or more genetic target locus has been altered by the CRISPR/Cas system, and which cells are transplanted in non-human eukaryote as a model system for tumor formation and tumor evolution. In particular in vivo genetic screening methods for identifying genes involved in tumorigenesis and metastasis are disclosed. The invention further relates to kits and components for practicing the methods, as well as materials obtainable by the methods, in particular tumor and metastasis samples and cells or cell lines derived therefrom. The invention also relates to diagnostic and therapeutic methods derived from the information obtained in the modeling methods.

公开(公告)号：US12110523B2

公开(公告)日：2024-10-08

申请号：US17526816

申请日：2021-11-15

申请人： Wisconsin Alumni Research Foundation

发明人： Ronald T. Raines ,  Kalie Mix

IPC分类号： C07C231/12 ,  C07C245/18 ,  C07C269/06 ,  C07C319/12 ,  C07D207/46 ,  C07K1/13 ,  C12N9/96

CPC分类号： C12N9/96 ,  C07C231/12 ,  C07C245/18 ,  C07C269/06 ,  C07C319/12 ,  C07D207/46 ,  C07K1/13 ,  C07C319/12 ,  C07C323/52 ,  C07C269/06 ,  C07C271/22 ,  C07C231/12 ,  C07C235/34

摘要： Methods and reagents for esterification of biological molecules including proteins, polypeptides and peptides. Diazo compounds of formula I:




where R is hydrogen, an alkyl, an alkenyl or an alkynyl, RA represents 1-5 substituents on the indicated phenyl ring and RM is an organic group. RM includes a label, a cell penetrating group, a cell targeting group, or a reactive group or latent reactive group for reaction to bond to a label, a cell penetrating group, or a cell targeting group, among other organic groups useful for esterification of biological molecules. Also provided are diazo compounds which are bifunctional and trifunctional coupling reagents as well as reagents for the synthesis of compounds of formula I.

公开(公告)号：US20240229003A1

公开(公告)日：2024-07-11

申请号：US18425681

申请日：2024-01-29

申请人： RESEARCH DEVELOPMENT FOUNDATION

发明人： Xinjian LIN ,  Xiying SHANG ,  Stephen B. HOWELL

IPC分类号： C12N9/64 ,  A61K47/65 ,  A61K47/68 ,  A61K48/00 ,  C07K14/435 ,  C07K14/59 ,  C12N9/52 ,  C12N9/96

CPC分类号： C12N9/6467 ,  A61K47/65 ,  A61K47/6889 ,  C07K14/43504 ,  C07K14/59 ,  C12N9/52 ,  C12N9/6424 ,  C12N9/96 ,  C12Y304/21079 ,  C12Y304/22007 ,  C12Y304/2207 ,  A61K48/00 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/33 ,  C07K2319/40 ,  C07K2319/55 ,  C07K2319/70 ,  C07K2319/74 ,  C07K2319/90

摘要： The invention is directed to cell-targeted cytotoxic agents, including sortase serine protease constructs. Methods for targeted cell killing for treatment of proliferative diseases, for example, cancer, are provided. Exemplary embodiments comprise an R-spondin ligand for targeting the cytotoxic agents to effect the cell killing.

公开(公告)号：US20240197810A1

公开(公告)日：2024-06-20

申请号：US18490182

申请日：2023-10-19

申请人： Kodiak Sciences Inc.

发明人： Stephen A. Charles ,  Daniel Victor Perlroth ,  Lane A. Clizbe ,  Didier G. Benoit ,  Wayne To

IPC分类号： A61K38/00 ,  A61K31/4745 ,  A61K38/18 ,  A61K38/46 ,  A61K39/395 ,  A61K47/54 ,  A61K47/58 ,  A61K47/68 ,  A61K49/00 ,  C07K16/00 ,  C08F8/32 ,  C08F230/02 ,  C08F230/08 ,  C12N9/16 ,  C12N9/96

CPC分类号： A61K38/00 ,  A61K31/4745 ,  A61K38/1816 ,  A61K38/465 ,  A61K39/395 ,  A61K47/544 ,  A61K47/58 ,  A61K47/6805 ,  A61K47/6811 ,  A61K49/0002 ,  A61K49/0043 ,  A61K49/0054 ,  C07K16/00 ,  C08F8/32 ,  C08F230/02 ,  C12N9/16 ,  C12N9/96 ,  C12Y301/03001 ,  C07K2317/21 ,  C07K2317/55 ,  C08F230/085 ,  C08F2438/01

摘要： The present invention provides random copolymers containing zwitterions and one or more functional agents, and methods of preparing such random copolymers.

公开(公告)号：US11993797B2

公开(公告)日：2024-05-28

申请号：US17546447

申请日：2021-12-09

申请人： Omrix Biopharmaceuticals Ltd.

发明人： Roberto Meidler ,  Oleg Belyaev ,  Liliana Bar ,  Israel Nur

IPC分类号： C12N9/96 ,  A61K9/19 ,  A61K38/48 ,  A61K47/26 ,  A61K47/42 ,  C12N9/74

CPC分类号： C12N9/96 ,  A61K9/19 ,  A61K38/4833 ,  A61K47/26 ,  A61K47/42 ,  C12N9/6429 ,  C12Y304/21005

摘要： Provided are methods for lyophilization of an aqueous thrombin solution, thrombin solutions for use in such lyophilization methods, and solid thrombin compositions produced by such methods.

公开(公告)号：US20240142435A1

公开(公告)日：2024-05-02

申请号：US18300014

申请日：2023-04-13

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Ho Lam Tang ,  Ho Man Holly Tang

IPC分类号： G01N33/50 ,  C12N9/96

CPC分类号： G01N33/5005 ,  C12N9/96 ,  C07K2319/03 ,  C07K2319/50 ,  C07K2319/60 ,  G01N2510/00

摘要： The present invention relates to the field of anastasis, i.e., the process of reversal of apoptosis. More specifically, the present invention provides methods and compositions useful for studying anastasis. The present invention also provides a biosensor comprising (a) a split transcription factor complex comprising one half of a split transcription factor linked to a transmembrane domain via an enzyme cleavable linker; (b) a split transcription factor comprising the other half of the split transcription factor linked to a MTS via an enzyme-cleavable linker; and (c) a reporter system comprising (1) a first nucleic acid encoding a site specific recombinase operably linked to the site specific sequence for the transcription factor; and (2) a second nucleic acid comprising a stop codon cassette flanked by site specific recombination sequences, wherein the split transcription factor is Gal 4 or split Q. In other embodiments, the recombinase is Cre or FLP.

公开(公告)号：US20240141305A1

公开(公告)日：2024-05-02

申请号：US18488316

申请日：2023-10-17

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Noura S. Abul-Husn ,  Omri Gottesman ,  Alexander Li ,  Xiping Cheng ,  Yurong Xin ,  Jesper Gromada ,  Frederick E. Dewey ,  Aris Baras ,  Alan Shuldiner

IPC分类号： C12N9/04 ,  A61K31/713 ,  A61K38/44 ,  A61K38/46 ,  A61K47/61 ,  A61P1/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90 ,  C12Q1/32 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q1/6883 ,  G01N33/50

CPC分类号： C12N9/0006 ,  A61K31/713 ,  A61K38/443 ,  A61K38/465 ,  A61K47/61 ,  A61P1/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N15/907 ,  C12Q1/32 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Y101/01051 ,  C12Y101/01062 ,  G01N33/5067 ,  A61K48/0066 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/531 ,  C12N2320/34 ,  C12Q2600/156

摘要： Provided are compositions related to HSD17B13 variants, including nucleic acid molecules and polypeptides related to variants of HSD17B13, and cells comprising those nucleic acid molecules and polypeptides. Also provided are methods related to HSD17B13 variants. Such methods include methods for detecting the presence of the HSD17B13 rs72613567 variant in a biological sample comprising genomic DNA, for detecting the presence or levels of any one of variant HSD17B13 Transcripts C, D, E, F, G, and H, and particularly D, in a biological sample comprising mRNA or cDNA, or for detecting the presence or levels of any one of variant HSD17B13 protein Isoforms C, D, E, F, G, or H, and particularly D, in a biological sample comprising protein. Also provided are methods for determining a subject's susceptibility to developing a liver disease or of diagnosing a subject with liver disease.

公开(公告)号：US11965191B2

公开(公告)日：2024-04-23

申请号：US17157880

申请日：2021-01-25

申请人： California Institute of Technology

发明人： Xiaojing Gao ,  Lucy S. Chong ,  Michael Elowitz ,  Mark William Budde ,  Matthew Sun-min Kim

IPC分类号： C12N9/50 ,  C12N9/06 ,  C12N9/96

CPC分类号： C12N9/506 ,  C12N9/003 ,  C12N9/96 ,  C12Y105/01003 ,  C12Y304/21098 ,  C12Y304/22044 ,  C07K2319/50

摘要： The present disclosure relates to systems, methods and compositions provided herein that include a compound protease. The compound protease can contain a protease domain, a cut site for another enzyme and an association domain. In some embodiments, the compound protease is part of a protein circuit.

公开(公告)号：US20240117382A1

公开(公告)日：2024-04-11

申请号：US18488197

申请日：2023-10-17

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： David Benjamin Turitz Cox ,  James E. Dahlman ,  Feng Zhang

IPC分类号： C12N15/90 ,  A61K38/43 ,  C12N9/96 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85

CPC分类号： C12N15/907 ,  A61K38/43 ,  C12N9/96 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/111 ,  C12N15/63 ,  C12N15/85 ,  C12N9/52 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3519

摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

公开(公告)号：US20240109845A1

公开(公告)日：2024-04-04

申请号：US18194410

申请日：2023-03-31

申请人： The Regents of the University of California

发明人： Kevan M. Shokat ,  Daniel Gentile ,  Steven Moss

IPC分类号： C07D231/14 ,  C12N9/14 ,  C12N9/96 ,  G01N33/68

CPC分类号： C07D231/14 ,  C12N9/14 ,  C12N9/96 ,  C12Y306/05002 ,  G01N33/68

摘要： Disclosed herein, inter alia, are compositions and methods for modulating Ras and treating cancer.