公开(公告)号：US20150183820A1

公开(公告)日：2015-07-02

申请号：US14416579

申请日：2013-07-29

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE TECHNOLOGY ,  DAICEL CORPORATION

Inventor： Shinya Honda ,  Hideki Watanabe ,  Chuya Yoshida ,  Momoko Ueda ,  Yasuto Nakai ,  Yutaka Isobe

IPC: C07K1/22 ,  C07K17/08 ,  C07K14/315

CPC classification number: C07K1/22 ,  C07K14/315 ,  C07K16/00 ,  C07K16/065 ,  C07K17/08 ,  C07K17/14

Abstract: The purpose of the present invention is to provide a superior protein with more decreased binding property to the Fc region of immunoglobulin and/or more decreased binding property to the Fab region thereof in the weakly acidic region in comparison with a protein containing an extracellular domain of a wild-type protein G without spoiling the high antibody binding property in the neutral region, and to allow the antibody to be easily captured and recovered without denaturing it by means of a capturing agent and column comprising the above protein.The present invention relates to a capturing agent for an antibody, immunoglobulin G or a protein comprising an Fc region of the immunoglobulin G, wherein a protein consisting of a tandem-type multimer of extracellular domain mutants that have binding property to the protein comprising the Fc region of the immunoglobulin G is immobilized to a water-insoluble solid support via mainly an amino group, and a column for a chromatography for separating and purifying the protein, which is filled with the capturing agent.

Abstract translation: 本发明的目的是提供与含有细胞外结构域的蛋白质相比，在弱酸性区域中提供具有更低的与免疫球蛋白Fc区的结合特性和/或更弱的与Fab区的结合特性的优异蛋白质 野生型蛋白G，而不破坏中性区域中的高抗体结合性质，并且允许抗体容易地捕获和回收，而不通过包含上述蛋白质的捕获剂和柱进行变性。 本发明涉及用于抗体，免疫球蛋白G或包含免疫球蛋白G的Fc区的蛋白质的捕获剂，其中由与包含Fc的蛋白质结合的细胞外结构域突变体的串联型多聚体组成的蛋白质 免疫球蛋白G的区域主要通过氨基固定在水不溶性固体支持物上，用于分离和纯化蛋白质的色谱柱，填充有捕获剂。