公开(公告)号：US20120052497A1

公开(公告)日：2012-03-01

申请号：US13159904

申请日：2011-06-14

Applicant: Win Den CHEUNG

Inventor： Win Den CHEUNG

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16 ,  Y02A50/451 ,  Y02A50/59

Abstract: Methods are described for the rapid, simultaneous and quantitative PCR detection of pathogenic Salmonella spp. and E. coli O157: H7 nucleic acid sequences in a sample in real-time. The detection method is fast, accurate and suitable for high throughput applications. Convenient, user-friendly and reliable diagnostic kits are also described for the simultaneous detection of Salmonella and E. coli O157: H7 in food samples and on surfaces.

Abstract translation: 描述了用于快速，同时和定量PCR检测致病性沙门氏菌的方法。 和大肠杆菌O157：H7核酸序列。 检测方法快速，准确，适合高通量应用。 还描述了方便，用户友好和可靠的诊断试剂盒，用于在食品样品和表面上同时检测沙门氏菌和大肠杆菌O157：H7。

公开(公告)号：US08524451B2

公开(公告)日：2013-09-03

申请号：US13046055

申请日：2011-03-11

Applicant: Jason Opdyke ,  Win Den Cheung ,  Jun Li

Inventor： Jason Opdyke ,  Win Den Cheung ,  Jun Li

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/689 ,  Y02A50/451 ,  Y02A50/59 ,  Y02P20/582

Abstract: A method is described for the real-time detection of Salmonella species in foods and on surfaces. Salmonella are enriched in media to increase their cell density prior to analysis. DNA is recovered by lysis in the presence of azide, proteinase K, and detergent. Real-time detection of Salmonella species is performed in a PCR reaction using gene specific primers and a cleavable chimeric fluorescent probe. The method also describes an internal control to confirm the efficiency of nucleic acid amplification and detection. The method is amenable to medium and high throughput analysis.

Abstract translation: 描述了一种在食物和表面上实时检测沙门氏菌物种的方法。 在分析之前，沙门氏菌富集培养基以增加其细胞密度。 在叠氮化物，蛋白酶K和洗涤剂的存在下通过裂解回收DNA。 使用基因特异性引物和可切割嵌合荧光探针在PCR反应中进行沙门氏菌物种的实时检测。 该方法还描述了核酸扩增和检测效率的内部控制。 该方法适用于中，高通量分析。

公开(公告)号：US20110223598A1

公开(公告)日：2011-09-15

申请号：US13046055

申请日：2011-03-11

Applicant: Jason OPDYKE ,  Win Den CHEUNG ,  Jun LI

Inventor： Jason OPDYKE ,  Win Den CHEUNG ,  Jun LI

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  Y02A50/451 ,  Y02A50/59 ,  Y02P20/582

Abstract: A method is described for the real-time detection of Salmonella species in foods and on surfaces. Salmonella are enriched in media to increase their cell density prior to analysis. DNA is recovered by lysis in the presence of azide, proteinase K, and detergent. Real-time detection of Salmonella species is performed in a PCR reaction using gene specific primers and a cleavable chimeric fluorescent probe. The method also describes an internal control to confirm the efficiency of nucleic acid amplification and detection. The method is amenable to medium and high throughput analysis.

Abstract translation: 描述了一种在食物和表面上实时检测沙门氏菌物种的方法。 在分析之前，沙门氏菌富集培养基以增加其细胞密度。 在叠氮化物，蛋白酶K和洗涤剂的存在下通过裂解回收DNA。 使用基因特异性引物和可切割嵌合荧光探针在PCR反应中进行沙门氏菌物种的实时检测。 该方法还描述了核酸扩增和检测效率的内部控制。 该方法适用于中，高通量分析。