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公开(公告)号：US08501457B2
公开(公告)日：2013-08-06
申请号：US13155469
申请日：2011-06-08
Applicant: Jun Li , Win Den Cheung
Inventor： Jun Li , Win Den Cheung
IPC: C12N1/20
CPC classification number: C12N1/20
Abstract: A medium for enriching Listeria spp. without polymerase chain reaction (PCR) inhibition and a method of using the medium.
Abstract translation: 一种富含李斯特菌属的培养基 没有聚合酶链反应（PCR）抑制和使用该介质的方法。
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公开(公告)号：US20120052555A1
公开(公告)日：2012-03-01
申请号：US13155469
申请日：2011-06-08
Applicant: Jun LI , Win Den CHEUNG
Inventor： Jun LI , Win Den CHEUNG
IPC: C12N1/20
CPC classification number: C12N1/20
Abstract: A medium for enriching Listeria spp. without polymerase chain reaction (PCR) inhibition and a method of using the medium.
Abstract translation: 一种富含李斯特菌属的培养基 没有聚合酶链反应（PCR）抑制和使用该介质的方法。
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3.发明申请OLIGONUCLEOTIDES FOR DETECTING E. coli O157:H7 STRAINS AND USE THEREOF 审中-公开检测大肠杆菌O157：H7菌株及其用途的寡核苷酸公开(公告)号：US20120052494A1
公开(公告)日：2012-03-01
申请号：US13109043
申请日：2011-05-17
Applicant: Jun LI , Win Den CHEUNG , Jason OPDYKE
Inventor： Jun LI , Win Den CHEUNG , Jason OPDYKE
IPC: C12Q1/68
CPC classification number: C12Q1/689
Abstract: Oligonucleotides, a kit, and a method for detecting E. coli O157:H7 strains are provided. According to the kit for detecting E. coli O157:H7 strains and the method of detecting E. coli O157:H7 strains by using the kit, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
Abstract translation: 提供寡核苷酸，试剂盒和检测大肠杆菌O157：H7菌株的方法。 根据用于检测大肠杆菌O157：H7菌株的试剂盒和使用试剂盒检测大肠杆菌O157：H7菌株的方法，可以用实际的样品数目减少的数量快速鉴定检测结果 -时间。
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公开(公告)号：US20110294674A1
公开(公告)日：2011-12-01
申请号：US13108311
申请日：2011-05-16
Applicant: Win Den CHEUNG , Jason OPDYKE
Inventor： Win Den CHEUNG , Jason OPDYKE
CPC classification number: C12Q1/686 , C12N9/22 , C12Q1/6848 , C12Q2521/327
Abstract: A reversibly modified ‘hot start’ RNAse H enzyme composition is described for the improved CATACLEAVE™ probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE™ probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE™ PCR in a single reaction mix.
Abstract translation: 描述了可逆修饰的“热启动”RNA酶H酶组合物，用于改进测试样品中核酸序列的CATACLEAVE™探针检测。 酶组合物的关键特征是在逆转录 - PCR循环过程中调节RNA酶H的催化活性的能力。 因此，可以最初抑制RNAse H活性以最小化RNA逆转录前的DNA引物异源双链体的降解。 在cDNA合成完成后，诱导RNAse H活性，促进CATACLEAVE™探针的切割和荧光检测，其与逆转录酶-PCR产物内的靶DNA序列退火。 诱导型RNA酶H酶适用于在单一反应混合物中需要一步逆转录酶CATACLEAVE™PCR的高通量应用。
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公开(公告)号：US08618253B2
公开(公告)日：2013-12-31
申请号：US13108311
申请日：2011-05-16
Applicant: Win Den Cheung , Jason Opdyke
Inventor： Win Den Cheung , Jason Opdyke
CPC classification number: C12Q1/686 , C12N9/22 , C12Q1/6848 , C12Q2521/327
Abstract: A reversibly modified ‘hot start’ RNAse H enzyme composition is described for the improved CATACLEAVE™ probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE™ probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE™ PCR in a single reaction mix.
Abstract translation: 描述了可逆修饰的“热启动”RNA酶H酶组合物，用于改进测试样品中核酸序列的CATACLEAVE TM探针检测。 酶组合物的关键特征是在逆转录 - PCR循环过程中调节RNA酶H的催化活性的能力。 因此，可以最初抑制RNAse H活性以最小化RNA逆转录前的DNA引物异源双链体的降解。 在cDNA合成完成后，诱导RNAse H活性以促进CATACLEAVE TM探针的切割和荧光检测，其与逆转录酶-PCR产物内的靶DNA序列退火。 诱导型RNA酶H酶适用于需要在单次反应混合物中进行一步逆转录酶CATACLEAVE TM PCR的高通量应用。
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Patent Agency Ranking
6.发明申请NUCLEIC ACID DETECTION BY OLIGONUCLEOTIDE PROBES CLEAVED BY BOTH EXONUCLEASE AND ENDONUCLEASE 审中-公开由外源核酸酶和内切酶清除的寡核苷酸探针的核酸检测公开(公告)号：US20130302794A1
公开(公告)日：2013-11-14
申请号：US13467586
申请日：2012-05-09
Applicant: Jun LI , Win Den CHEUNG , Jason OPDYKE , John HARVEY , Songchun CHONG
Inventor： Jun LI , Win Den CHEUNG , Jason OPDYKE , John HARVEY , Songchun CHONG
IPC: C12Q1/68
CPC classification number: C12Q1/706 , C12Q1/6851 , C12Q1/689 , C12Q2521/301 , C12Q2521/319 , C12Q2521/327 , C12Q2565/1015
Abstract: Disclosed is a method in the fields of biochemistry and molecular biology. The method is related to improve cleavage kinetics of labeled oligonucleotide probes and, consequently, increases signal-to-noise ratio in detecting nucleic acids.
Abstract translation: 公开了生物化学和分子生物学领域的一种方法。 该方法与提高标记的寡核苷酸探针的切割动力学有关，因此增加检测核酸中的信噪比。
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7.发明申请KIT FOR DETECTING CHLAMYDIA TRACHOMATIS STRAINS AND METHOD FOR DETECTING CHLAMYDIA TRACHOMATIS STRAINS USING THE SAME 审中-公开用于检测氯霉素漂白菌菌株的试剂盒和使用该酶菌株检测氯霉素TRACHOMATIS菌株的方法公开(公告)号：US20120052500A1
公开(公告)日：2012-03-01
申请号：US13159962
申请日：2011-06-14
Applicant: Win Den CHEUNG
Inventor： Win Den CHEUNG
IPC: C12Q1/68
CPC classification number: C12Q1/689
Abstract: A kit for detecting Chlamydia trachomatis in a test sample is disclosed. In addition a method is described for the real-time detection of Chlamydia trachomatis in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
Abstract translation: 公开了一种用于在测试样品中检测沙眼衣原体的试剂盒。 另外还描述了使用试剂盒在测试样品中实时检测沙眼衣原体的方法。 根据检测方法，能够以实时的样本数量减少的方式快速识别出检测结果。
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8.发明申请公开(公告)号：US20120045750A1
公开(公告)日：2012-02-23
申请号：US13160054
申请日：2011-06-14
Applicant: Win Den CHEUNG
Inventor： Win Den CHEUNG
IPC: C12Q1/70
CPC classification number: C12Q1/703 , C12Q2600/16 , C12Q2521/327 , C12Q2525/121
Abstract: A kit for detecting HIV-2 strains in a test sample is disclosed. In addition a method is described for the real-time detection of HIV-2 strains in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
Abstract translation: 公开了一种用于在测试样品中检测HIV-2菌株的试剂盒。 此外，还描述了使用试剂盒在测试样品中实时检测HIV-2毒株的方法。 根据检测方法，能够以实时的样本数量减少的方式快速识别出检测结果。
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9.发明申请KIT FOR DETECTING HEPATITIS B VIRUS AND METHOD FOR DETECTING HEPATITIS B VIRUS USING THE SAME 审中-公开用于检测乙型肝炎病毒的试剂盒和使用其检测乙型肝炎病毒的方法公开(公告)号：US20120045747A1
公开(公告)日：2012-02-23
申请号：US13117368
申请日：2011-05-27
Applicant: Win Den CHEUNG
Inventor： Win Den CHEUNG
IPC: C12Q1/70
CPC classification number: C12Q1/706
Abstract: A kit for detecting HBV in a test sample is disclosed. In addition a method is described for the real-time detection of HBV in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
Abstract translation: 公开了一种用于在测试样品中检测HBV的试剂盒。 此外，还描述了使用试剂盒在测试样品中实时检测HBV的方法。 根据检测方法，能够以实时的样本数量减少的方式快速识别出检测结果。
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10.发明授权公开(公告)号：US09157128B2
公开(公告)日：2015-10-13
申请号：US13160054
申请日：2011-06-14
Applicant: Win Den Cheung
Inventor： Win Den Cheung
CPC classification number: C12Q1/703 , C12Q2600/16 , C12Q2521/327 , C12Q2525/121
Abstract: A kit for detecting HIV-2 strains in a test sample is disclosed. In addition a method is described for the real-time detection of HIV-2 strains in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
Abstract translation: 公开了一种用于在测试样品中检测HIV-2菌株的试剂盒。 此外，还描述了使用试剂盒在测试样品中实时检测HIV-2毒株的方法。 根据检测方法，能够以实时的样本数量减少的方式快速识别出检测结果。
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