公开(公告)号：US20240360520A1

公开(公告)日：2024-10-31

申请号：US18354652

申请日：2023-07-19

Applicant: Fudan University

Inventor： SUHUA ZHANG ,  CHENGTAO LI

IPC: C12Q1/6888

CPC classification number: C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: A primer composition, a kit and a method for detecting polymorphic STR markers based on capillary electrophoresis and applications thereof are provided, which are used to simultaneously amplify 13 STR loci on cattle genome. The primer composition includes one or more pairs of primers with sequences as shown in SEQ ID NO: 1˜26. Developmental validation indicated that the kit is of high sensitivity, fine specificity, strong stability and high accuracy. This provides an effective tool for species identification, individual identification and parental analysis of cattle samples in civil disputes and illegal crimes.

公开(公告)号：US12123061B2

公开(公告)日：2024-10-22

申请号：US16339968

申请日：2017-10-04

Applicant: National University of Ireland, Galway

Inventor： Thomas Barry ,  Kate Reddington ,  Elizabeth Minogue

IPC: C12Q1/68 ,  C12Q1/689

CPC classification number: C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/16 ,  Y02A50/30

Abstract: This invention relates to the detection of Legionella, more specifically Legionella pneumophila, more specifically Legionella pneumophila serogroup 1, in a sample. Methods of detection, and assays and kits useful for detection, are also disclosed. The invention provides a method for identifying the presence, absence, or quantity of Legionella pneumophila serogroup 1 in a sample, the method comprising the steps of detecting the presence, absence, or quantity of at least part of the nucleic acid sequence of the lpg0768 gene or an expression product thereof, and correlating the presence, absence, or quantity of the at least part of the nucleic acid sequence of the lpg0768 gene or an expression product thereof, to the presence, absence, or quantity of Legionella pneumophila serogroup 1 in the sample.

公开(公告)号：US20240327926A1

公开(公告)日：2024-10-03

申请号：US18607277

申请日：2024-03-15

Applicant: Foundation Medicine, Inc.

Inventor： Geoffrey Alan OTTO ,  Michelle NAHAS ,  Doron LIPSON

IPC: C12Q1/6886 ,  C12N15/10 ,  C12Q1/6806 ,  G16B25/00 ,  G16B25/10 ,  G16B30/00 ,  G16B30/10 ,  G16B45/00

CPC classification number: C12Q1/6886 ,  C12N15/1065 ,  C12Q1/6806 ,  G16B25/00 ,  G16B25/10 ,  G16B30/00 ,  G16B30/10 ,  G16B45/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Methods of evaluating or providing a clonal profile of a subject interval, e.g., a subgenomic interval, or an expressed subgenomic interval (or of a cell containing the same), in a subject, are disclosed.

公开(公告)号：US12104205B2

公开(公告)日：2024-10-01

申请号：US16624782

申请日：2017-06-20

Applicant: MGI Tech Co., Ltd.

Inventor： Lin Yang ,  Haojun Jiang ,  Peng Zeng ,  Xuehan Zhuang ,  Ya Gao ,  Yanyan Zhang ,  Hui Jiang ,  Jing Guo ,  Fang Chen ,  Xun Xu

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q2600/16

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

公开(公告)号：US20240301485A1

公开(公告)日：2024-09-12

申请号：US18660998

申请日：2024-05-10

Applicant: New England Biolabs, Inc.

Inventor： Ivan R. Correa, JR. ,  Eric Wolf ,  Nan Dai ,  Erbay Yigit ,  Sebastian Grünberg

IPC: C12Q1/6872 ,  C12Q1/6806

CPC classification number: C12Q1/6872 ,  C12Y301/13005 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g., Homo sapiens, Escherichia coli, Aspergillus oryzae, Momordica charantia, Pyrococcus furiosus, Cucumis sativus, and Sus scrofa) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.

公开(公告)号：US12083512B2

公开(公告)日：2024-09-10

申请号：US16069824

申请日：2017-01-13

Applicant: European Molecular Biology Laboratory

Inventor： Hongxing Hu ,  Samantha Seah ,  Christoph A. Merten

IPC: B01L3/00 ,  C07K16/28 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C40B20/08

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  C07K16/28 ,  C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/686 ,  B01L2200/0647 ,  B01L2300/021 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  C40B20/08 ,  C12Q1/6846 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

公开(公告)号：US12076381B2

公开(公告)日：2024-09-03

申请号：US17576185

申请日：2022-01-14

Applicant: Immatics Biotechnologies GmbH

Inventor： Andrea Mahr ,  Toni Weinschenk ,  Oliver Schoor ,  Jens Fritsche ,  Harpreet Singh ,  Phillip Mueller ,  Julia Leibold ,  Valentina Goldfinger

IPC: A61K39/00 ,  C07K14/47 ,  C07K14/725 ,  C07K16/30 ,  C12N5/0783 ,  C12N15/115 ,  C12Q1/6886 ,  G01N33/574 ,  G01N33/68 ,  A61K38/00

CPC classification number: A61K39/0011 ,  C07K14/47 ,  C07K14/4748 ,  C07K14/7051 ,  C07K16/30 ,  C12N5/0636 ,  C12N15/115 ,  C12Q1/6886 ,  G01N33/5748 ,  G01N33/6848 ,  A61K38/00 ,  A61K2039/5158 ,  A61K2039/572 ,  C07K2319/00 ,  C12N2310/16 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/57407 ,  G01N2570/00 ,  G01N2800/52

Abstract: The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.

公开(公告)号：US20240279738A1

公开(公告)日：2024-08-22

申请号：US18463607

申请日：2023-09-08

Applicant: The General Hospital Corporation ,  GPB Scientific, LLC ,  Verinata Health, Inc.

Inventor： Daniel Shoemaker ,  Mehmet Toner ,  Ravi Kapur ,  Roland B. Stoughton ,  Ronald W. Davis

IPC: C12Q1/6883 ,  B01L3/00 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6881 ,  G01N15/10

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6881 ,  B01L3/502761 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N2015/1006 ,  G01N2015/1029 ,  Y10T436/143333

Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

公开(公告)号：US12065705B2

公开(公告)日：2024-08-20

申请号：US15733628

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Looney

IPC: C40B30/04 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16 ,  C40B30/04

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor and/or B cell receptor repertoires in samples prior to a treatment and predicting a subjects response to the treatment based on the measured convergence frequency. In another aspect, methods provide for an immune receptor haplotype group and predicting a subjects potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.

公开(公告)号：US12054794B2

公开(公告)日：2024-08-06

申请号：US17840835

申请日：2022-06-15

Applicant: Fushi Wen ,  Frederick H. Eggers ,  Michael E. Hogan

Inventor： Fushi Wen ,  Frederick H. Eggers ,  Michael E. Hogan

IPC: C12Q1/70 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/6853 ,  C12Q1/689

CPC classification number: C12Q1/701 ,  C12Q1/6837 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q1/689 ,  C12Q2600/16

Abstract: Provided herein is a method for detecting the presence of a COVID-19 virus RNA or other pathogenic respiratory viruses, such as an influenza virus, or other RNA of interest in a sample. Nucleic acids are obtained from the sample and are used as a template in a combined isothermal reverse transcription, RNAse H and isothermal amplification reaction to generate single stranded RNA amplicons containing sequences complementary to fluorescent labeled detector probes. The single-stranded RNA amplicons hybridize to the detector probe and to hybridization probes with sequences complementary to a sequence determinant in the COVID-19 or other virus RNAs. The microarray is imaged to detect fluorescent signals thereby identifying the virus.