公开(公告)号：US20230271190A1

公开(公告)日：2023-08-31

申请号：US18175885

申请日：2023-02-28

Applicant: 10x Genomics, Inc.

Inventor： Hendricus MARINDRA ,  Denis Pristinski ,  David Morgan ,  Siyuan Xing

IPC: B01L9/00

CPC classification number: B01L9/00 ,  B01L2200/025 ,  B01L2200/04

Abstract: Devices (e.g., flow cell holders) and methods for flow cell assembly and disassembly are provided. The flow cell holders and methods can be used to join or separate the bottom and top layers of a flow cell.

公开(公告)号：US20230264186A1

公开(公告)日：2023-08-24

申请号：US18151344

申请日：2023-01-06

Applicant: 10x Genomics, Inc.

Inventor： Bill Kengli LIN ,  Siyuan Xing ,  Hanyoup Kim

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/16 ,  B01L2300/18 ,  B01L2300/161 ,  B01L2300/0645 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0677

Abstract: Disclosed herein are devices and methods for contacting a tissue sample with liquids to perform a variety of processes and analyses. In various embodiments, the device includes a hydrophilic flow path defined at least in part by a surface pattern (e.g., bounded by hydrophobic regions). The flow path includes a sample area sized to receive a biological sample. The flow path includes at least one valve configured to control flow in the flow path in response to an external stimulus.

公开(公告)号：US20230017773A1

公开(公告)日：2023-01-19

申请号：US17873586

申请日：2022-07-26

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  G01N1/31 ,  C12Q1/6841

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.