公开(公告)号：US20170355750A1

公开(公告)日：2017-12-14

申请号：US15619835

申请日：2017-06-12

Applicant: Academia Sinica

Inventor： An-Suei YANG ,  Hong-Sen CHEN ,  Chung-Ming YU ,  Shin-Chen HOU ,  Wei-Ying KUO ,  Yi-Kai CHIU ,  Yueh-Liang TSOU ,  Hung-Ju HSU ,  Hwei-Jiung WANG ,  Shih-Hsien CHUANG ,  Chao-Pin LEE

IPC: C07K16/00 ,  C07K16/32 ,  C07K19/00 ,  A61K39/00

Abstract: Disclosed herein are methods for high-throughput screening of a functional antibody fragment for an immunoconjugate that targets a protein antigen. The method combines a phage-displayed synthetic antibody library and high-throughput cytotoxicity screening of non-covalently assembled immunotoxins or cytotoxic drug to identify highly functional synthetic antibody fragments for delivering toxin payloads.