公开(公告)号：US10266857B2

公开(公告)日：2019-04-23

申请号：US15955267

申请日：2018-04-17

Applicant: AJINOMOTO CO., INC.

Inventor： Jun Takakura ,  Hiroyuki Nozaki

IPC: C12P13/04 ,  C12P13/08 ,  C12N9/80

Abstract: The present invention provides a method for producing Nε-dodecanoyl-L-lysine using an enzyme having properties suitable for the industrial production of Nε-dodecanoyl-L-lysine. More specifically, the present invention provides a method for producing Nε-acyl-L-lysine including reacting a carboxylic acid or a salt thereof and L-lysine or a salt thereof in the presence of a protein having an amino acid sequence of SEQ ID NO: 1; a protein having an amino acid sequence in which one or several amino acid residues are inserted, added, deleted, or substituted in the amino acid sequence of SEQ ID NO: 1 and having Nε-acyl-L-lysine-specific aminoacylase activity; and a protein which has 90% or higher homology with the amino acid sequence of SEQ ID NO: 1 and having Nε-acyl-L-lysine-specific aminoacylase activity.

公开(公告)号：US20180230502A1

公开(公告)日：2018-08-16

申请号：US15955267

申请日：2018-04-17

Applicant: AJINOMOTO CO., INC.

Inventor： Jun Takakura ,  Hiroyuki Nozaki

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12N9/80 ,  C12Y305/01014

Abstract: The present invention provides a method for producing Nε-dodecanoyl-L-lysine using an enzyme having properties suitable for the industrial production of Nε-dodecanoyl-L-lysine. More specifically, the present invention provides a method for producing Nε-acyl-L-lysine including reacting a carboxylic acid or a salt thereof and L-lysine or a salt thereof in the presence of a protein having an amino acid sequence of SEQ ID NO: 1; a protein having an amino acid sequence in which one or several amino acid residues are inserted, added, deleted, or substituted in the amino acid sequence of SEQ ID NO: 1 and having Nε-acyl-L-lysine-specific aminoacylase activity; and a protein which has 90% or higher homology with the amino acid sequence of SEQ ID NO: 1 and having Nε-acyl-L-lysine-specific aminoacylase activity.

公开(公告)号：US20160340707A1

公开(公告)日：2016-11-24

申请号：US15234119

申请日：2016-08-11

Applicant: AJINOMOTO CO., INC.

Inventor： Chihiro TSUJI ,  Hiroyuki Nozaki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02 ,  C12N15/70

Abstract: γ-Glu-Val synthetase suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. By using γ-Glu-Val synthetase showing a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of 2.0 or higher, such as γ-Glu-Val synthetase of Kocuria rosea (AJ3132), γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

Abstract translation: 适用于产生γ-Glu-Val的γ-Glu-Val合成酶，以及使用其生产γ-Glu-Val-Gly的方法。 通过使用显示γ-谷氨酰缬氨酸合成酶活性与γ-谷氨酰甘氨酸合成酶活性比为2.0以上的γ-Glu-Val合成酶，例如蔷薇科（AJ3132）的γ-Glu-Val合成酶，γ-Glu-Val-Gly 由Glu，Val和Gly作为原料生产。