公开(公告)号：US10508295B2

公开(公告)日：2019-12-17

申请号：US15234119

申请日：2016-08-11

Applicant: AJINOMOTO CO., INC.

Inventor： Chihiro Tsuji ,  Hiroyuki Nozaki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02 ,  C12N15/70

Abstract: γ-Glu-Val synthetase suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. By using γ-Glu-Val synthetase showing a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of 2.0 or higher, such as γ-Glu-Val synthetase of Kocuria rosea (AJ3132), γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

公开(公告)号：US09580696B2

公开(公告)日：2017-02-28

申请号：US14243458

申请日：2014-04-02

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki Nozaki ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/10 ,  C07K5/093 ,  C07K5/02

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Abstract translation: 一种γ-Glu-Val-Gly的制造方法，其特征在于，在γ-谷氨酰基转移酶，含有该微生物的微生物或其加工物的存在下，使Val-Gly与γ-谷氨酰供体反应， Glu-Val-Gly，其中γ-谷氨酰转移酶由大亚基和小亚基组成，小亚基具有特异性突变。

公开(公告)号：US20220251611A1

公开(公告)日：2022-08-11

申请号：US17730360

申请日：2022-04-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yasuaki Takakura ,  Takuto Ono ,  Olga Igonina ,  Keiko Koenuma ,  Hiroyuki Nozaki

IPC: C12P7/24

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced by a method including a step of using amino acid deaminase (AAD), 4-hydroxymandelate synthase (HMAS), (S)-mandelate dehydrogenase (SMDH), and benzoylformate decarboxylase (BFDC) over a time period, wherein the producing step is carried out in the presence of catalase during a portion of said time period.

公开(公告)号：US10113161B2

公开(公告)日：2018-10-30

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako Sasahara ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C12N9/00 ,  C07K5/02

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

公开(公告)号：US20160326510A1

公开(公告)日：2016-11-10

申请号：US15223662

申请日：2016-07-29

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Eri Tabuchi ,  Hideyuki Suzuki ,  Uno Tagami ,  Tatsuki Kashiwagi ,  Takayuki Ito ,  Hiroyuki Nozaki ,  Shunichi Suzuki

IPC: C12N9/00 ,  C07K5/02 ,  C12P21/02

CPC classification number: C12N9/93 ,  C07K5/0215 ,  C12P21/02 ,  C12Y603/02002

Abstract: A mutant glutamate-cysteine ligase (GSHA) suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and γ-Glu-Val-Gly is further produced by using γ-Glu-Val and Gly as raw materials. γ-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Abstract translation: 提供适用于产生γ-Glu-Val的突变型谷氨酸 - 半胱氨酸连接酶（GSHA），以及使用其制备γ-Glu-Val-Gly的方法。 通过使用与Glu和Val作为原料具有特异性突变的突变体GSHA，通过使用γ-Glu-Val和Gly作为原料进一步制备γ-Glu-Val-Gly来产生γ-Glu-Val。 通过使用与Glu，Val和Gly作为原料具有特异性突变的突变体GSHA来产生γ-Glu-Val-Gly。

公开(公告)号：US09677106B2

公开(公告)日：2017-06-13

申请号：US15235311

申请日：2016-08-12

Applicant: Ajinomoto Co., Inc.

Inventor： Hiroyuki Nozaki ,  Isao Abe ,  Jun Takakura ,  Rie Takeshita ,  Hideyuki Suzuki ,  Shunichi Suzuki

IPC: C12P21/02 ,  C07K5/093 ,  C12N9/10 ,  C12N15/70 ,  C07K5/02

CPC classification number: C12P21/02 ,  C07K5/0215 ,  C07K5/0819 ,  C12N9/104 ,  C12N15/70 ,  C12Y203/02002 ,  Y02P20/52

Abstract: A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

公开(公告)号：US12281340B2

公开(公告)日：2025-04-22

申请号：US17025173

申请日：2020-09-18

Applicant: AJINOMOTO CO., INC.

Inventor： Hidemi Nagata ,  Keiko Danjou ,  Jun Takakura ,  Hiroyuki Nozaki

IPC: C12P13/00 ,  C12N9/00 ,  C12N9/42 ,  C12N9/80 ,  C12P21/00

Abstract: A method efficiently produces a compound containing an N-acyl-amino group by an enzymatic method. Specifically, a method of producing a compound containing an N-acyl-amino group includes producing the compound containing an N-acyl-amino group by reacting a compound containing an amino group with a compound containing a carboxyl group in the presence of an enzyme having an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond.

公开(公告)号：US11788109B2

公开(公告)日：2023-10-17

申请号：US15911731

申请日：2018-03-05

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako Sasahara ,  Takayuki Ito ,  Hiroyuki Nozaki

IPC: C12P21/02 ,  C12N1/20 ,  C07K5/083 ,  C07K5/093 ,  C12N9/00 ,  C12N15/09 ,  C12R1/19

CPC classification number: C12P21/02 ,  C07K5/0806 ,  C07K5/0819 ,  C12N1/205 ,  C12N9/93 ,  C12N15/09 ,  C12R2001/19 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDIQ is reduced as an expression host, γ-Glu-Val-Gly is produced (Yom Glu, Val, and Gly as raw materials.

公开(公告)号：US20210071214A1

公开(公告)日：2021-03-11

申请号：US17025173

申请日：2020-09-18

Applicant: AJINOMOTO CO., INC.

Inventor： Hidemi Nagata ,  Keiko Danjou ,  Jun Takakura ,  Hiroyuki Nozaki

IPC: C12P13/00 ,  C12P21/00 ,  C12N9/42 ,  C12N9/00 ,  C12N9/80

Abstract: A method efficiently produces a compound containing an N-acyl-amino group by an enzymatic method. Specifically, a method of producing a compound containing an N-acyl-amino group includes producing the compound containing an N-acyl-amino group by reacting a compound containing an amino group with a compound containing a carboxyl group in the presence of an enzyme having an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond.

公开(公告)号：US20240240167A1

公开(公告)日：2024-07-18

申请号：US18618330

申请日：2024-03-27

Applicant: AJINOMOTO CO., INC.

Inventor： Mizuki Tamori ,  Uno Tagami ,  Hiroyuki Nozaki

IPC: C12N9/48 ,  C12P21/02

CPC classification number: C12N9/485 ,  C12P21/02

Abstract: Described herein is an enzyme useful for producing a target peptide, capable of producing the target peptide without requiring ATP. More specifically, provided herein is a modified enzyme includes an amino acid sequence such as a modified amino acid sequence including a mutation of one or more of the following amino acid residues: E81, I127, I136, T139, F140, G142, W143, I147, I181, I201, Q219, T229, M244, A249, P255, E256, I260, S293, N294, Y295, and I299 in an amino acid sequence of SEQ ID NO: 1, and



having the following property (a) or (b) enhanced relative to an enzyme of the amino acid sequence of SEQ ID NO: 1:
(a) imidazole dipeptide production activity; or
(b) thermal stability.