公开(公告)号：US20240001338A1

公开(公告)日：2024-01-04

申请号：US18360870

申请日：2023-07-28

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  EDWIN LOZANO

IPC: B01J20/285 ,  B01J20/289 ,  B01J20/30 ,  C07K1/16 ,  B01D15/38 ,  B01J20/28

CPC classification number: B01J20/285 ,  B01J20/289 ,  B01J20/3042 ,  C07K1/165 ,  B01D15/3847 ,  B01D15/3809 ,  B01J20/28085 ,  B01J2220/52

Abstract: The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-dioxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.