公开(公告)号：US20160046663A1

公开(公告)日：2016-02-18

申请号：US14720331

申请日：2015-05-22

Applicant: Case Western Reserve University

Inventor： Gary E. Wnek ,  Lenghui Meng

IPC: C07K1/107 ,  D01F1/10 ,  D01F4/00 ,  C07K14/78

CPC classification number: C07K1/1075 ,  A61L27/22 ,  C07K1/107 ,  C07K1/36 ,  C07K14/78 ,  D01F1/10 ,  D01F4/00

Abstract: A method of forming a cross-linked protein structures includes preparing a solution of protein dissolved in a benign solvent and forming an intermediate protein structure from the solution. The intermediate protein structure can be cross-linked by providing for a specific ratio of chemical cross-linking agents to form the cross-linked protein structure. The solution can be prepared by adding a cross-linker of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) at a ratio of two-to-one of NHS to EDC to alcohol. PBS buffer (20×) can be added to the solution until the volume ratio of PBS buffer (20×) to alcohol is about one-to-one. About 16 percent by weight of protein can be dissolved in the solution. The solution can be electrospun to form an intermediate protein structure. After a period of time, the protein structure can be cross-linked to form the cross-linked protein structure.

Abstract translation: 形成交联蛋白质结构的方法包括制备溶解在良溶剂中的蛋白质溶液并从溶液形成中间体蛋白质结构。 通过提供特定比例的化学交联剂以形成交联的蛋白质结构，可以交联中间体蛋白质结构。 该溶液可以通过以NHS与EDC的二对比与乙醇的比例加入N-羟基琥珀酰亚胺（NHS）和1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐（EDC）的交联剂来制备 。 可以向溶液中加入PBS缓冲液（20×），直到PBS缓冲液（20×）与醇的体积比约为1比1。 约16重量％的蛋白质可以溶解在溶液中。 该溶液可以静电纺丝以形成中间体蛋白质结构。 一段时间后，蛋白质结构可以交联形成交联的蛋白质结构。