利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.042 秒)

    Biochemical method to measure niacin status in a biological sample
    11.
    发明授权
    Biochemical method to measure niacin status in a biological sample 有权
    生物化学方法测定生物样品中的烟酸状态

    公开(公告)号：US06287796B1

    公开(公告)日：2001-09-11

    申请号：US09334782

    申请日：1999-06-16

    申请人： Elaine L. Jacobson ,  Myron K. Jacobson

    发明人： Elaine L. Jacobson ,  Myron K. Jacobson

    IPC分类号： C12Q132

    CPC分类号： C12Q1/32 ,  G01N33/82

    摘要： The present invention is a novel and rapid assay for intracellular niacin status in a biological sample that is both highly sensitive and accurate over a wide range of niacin status. The assay can be performed directly on a biological sample such as blood and is useful for determining the niacin status in a subject such as a human. In a preferred form, the assay is incorporated into a kit or an apparatus for the rapid and accurate determination of niacin status in a subject.

    摘要翻译： 本发明是一种新颖而快速的生物样品中细胞内烟酸状态的检测方法，它在广泛的烟酸状态下既高度灵敏又准确。 该测定可以直接在诸如血液的生物样品上进行，并且可用于确定受试者例如人的烟酸状态。 在优选形式中，将测定法并入试剂盒或用于快速和准确测定受试者中烟酸状态的装置中。

    Method for enhancing protective cellular responses to genotoxic stress in skin
    13.
    发明授权
    Method for enhancing protective cellular responses to genotoxic stress in skin 有权
    增强对皮肤基因毒性应激的保护性细胞反应的方法

    公开(公告)号：US07425319B2

    公开(公告)日：2008-09-16

    申请号：US10256403

    申请日：2002-09-27

    申请人： Elaine L. Jacobson ,  Myron K. Jacobson

    发明人： Elaine L. Jacobson ,  Myron K. Jacobson

    IPC分类号： A61K7/42 ,  A61K7/00 ,  A61K31/44

    CPC分类号： A61K31/00 ,  A61K8/4926 ,  A61K8/675 ,  A61K31/445 ,  A61Q19/00 ,  A61K2300/00

    摘要： The present invention is directed to methods of using pro-NAD agents capable of enhancing the dermal and epidermal skin cell NAD content. These pro-NAD agents may be administered topically, orally, or parenterally to enhance DNA repair and other protective responses to DNA damage. The invention further relates to pharmaceutical compositions comprising pro-NAD agents that effectively elevate intracellular NAD content. Finally, the invention relates to the method of using the pro-NAD agents to treat disorders such as sunburn and other skin deterioration that results from DNA damage in skin cells.

    摘要翻译： 本发明涉及使用能够增强皮肤和表皮皮肤细胞NAD含量的前体NAD试剂的方法。 这些pro-NAD药物可以局部，口服或肠胃外给药以增强DNA修复和对DNA损伤的其它保护性反应。 本发明进一步涉及包含有效提高细胞内NAD含量的原NAD代谢物的药物组合物。 最后，本发明涉及使用原NAD活性剂治疗由皮肤细胞中DNA损伤引起的晒伤和其它皮肤恶化的病症的方法。

    Genes encoding several poly (ADP-ribose) glycohydrolase (PARG) enzymes, the proteins and fragments thereof, and antibodies immunoreactive therewith
    17.
    发明授权
    Genes encoding several poly (ADP-ribose) glycohydrolase (PARG) enzymes, the proteins and fragments thereof, and antibodies immunoreactive therewith 有权
    编码几种聚（ADP-核糖）糖水解酶（PARG）酶的基因，其蛋白质及其片段和与其免疫反应的抗体

    公开(公告)号：US06337202B1

    公开(公告)日：2002-01-08

    申请号：US09511477

    申请日：2000-02-23

    申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

    发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

    IPC分类号： C12N924

    CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

    摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

    摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述了含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。