公开(公告)号：US06906180B2

公开(公告)日：2005-06-14

申请号：US09973451

申请日：2001-10-09

申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

IPC分类号： C12N9/24 ,  C12Q1/34 ,  C07K16/00

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述了含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。

公开(公告)号：US20070136833A1

公开(公告)日：2007-06-14

申请号：US11638012

申请日：2006-12-13

申请人： Myron Jacobson ,  Elaine Jacobson ,  Jean-Christophe Ame ,  Winston Lin

发明人： Myron Jacobson ,  Elaine Jacobson ,  Jean-Christophe Ame ,  Winston Lin

IPC分类号： A01K67/027 ,  C12Q1/68 ,  C07H21/02 ,  A61K48/00 ,  C12P21/06 ,  A61K39/395 ,  C12N9/22 ,  C07H21/04

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述了含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。

公开(公告)号：US20050176050A1

公开(公告)日：2005-08-11

申请号：US11058995

申请日：2005-02-15

申请人： Myron Jacobson ,  Elaine Jacobson ,  Jean-Christophe Ame ,  Winston Lin

发明人： Myron Jacobson ,  Elaine Jacobson ,  Jean-Christophe Ame ,  Winston Lin

IPC分类号： C12N9/24 ,  C12Q1/34 ,  C12Q1/68 ,  A61K48/00 ,  C07H21/04 ,  C12N9/22

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

公开(公告)号：US06677442B1

公开(公告)日：2004-01-13

申请号：US09698286

申请日：2000-10-30

申请人： Zhigang Wang ,  Wensheng Winston Lin ,  Hua Xin ,  Xiaohua Wu

发明人： Zhigang Wang ,  Wensheng Winston Lin ,  Hua Xin ,  Xiaohua Wu

IPC分类号： C12N1552

CPC分类号： C12N9/1241

摘要： The present invention relates to a human cDNA homologous to the yeast REV1 gene. The sequence of human REV1 (hREV1) gene is described.

摘要翻译： 本发明涉及与酵母REV1基因同源的人cDNA。 描述人REV1（hREV1）基因的序列。

公开(公告)号：US20070181474A1

公开(公告)日：2007-08-09

申请号：US11346355

申请日：2006-02-03

申请人： Winston Lin

发明人： Winston Lin

IPC分类号： C02F3/06

CPC分类号： C02F3/06 ,  C02F3/103 ,  Y02W10/15

摘要： A filter for a microbe reactor of sewage treatment installed in the microbe reactor is provided with innumerable irregular ringed filaments twisted together and piled up mutually and innumerable interspaces formed among the irregular ringed filaments. Each irregular ringed filament is provided with lots of recesses all over the surface for natural microbes to attach and proliferate thereon, so as to decompose organic compounds in the sewage effectively.

摘要翻译： 安装在微生物反应器中的用于污水处理微生物反应器的过滤器设置有无数的不规则环状细丝，它们在不规则环状长丝之间形成的相互无数的间隙相互缠绕在一起堆积。 每个不规则环状细丝在整个表面上设置有许多凹陷，用于天然微生物附着和增殖，从而有效地分解污水中的有机化合物。

公开(公告)号：US06333148B1

公开(公告)日：2001-12-25

申请号：US09302812

申请日：1999-04-30

申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

IPC分类号： C12Q168

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。

公开(公告)号：US07538194B2

公开(公告)日：2009-05-26

申请号：US11638012

申请日：2006-12-13

申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

IPC分类号： C12Q1/68

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

公开(公告)号：US20070104938A1

公开(公告)日：2007-05-10

申请号：US11269755

申请日：2005-11-09

申请人： Winston Lin

发明人： Winston Lin

IPC分类号： B32B3/26

CPC分类号： C08J9/04 ,  B29C44/28 ,  B29C44/462 ,  C08J9/24 ,  C08J2327/06 ,  Y10T428/249953

摘要： A foam cushion with a high water-absorption ability is made mainly from PVC powder and full of open air cells. The air cells in the foam cushion are broken to connect with one another and those on the surface of the foam cushion are broken to connect with open air. Thus, the foam cushion provides a good elastic buffering and high water-absorption ability.

摘要翻译： 具有高吸水能力的泡沫垫主要由PVC粉末和充满露天电池制成。 泡沫垫中的气泡被破碎以相互连接，并且泡沫垫表面上的气泡被破坏以与露天连接。 因此，泡沫垫提供良好的弹性缓冲和高吸水能力。

公开(公告)号：US06395543B1

公开(公告)日：2002-05-28

申请号：US09511507

申请日：2000-02-23

申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

IPC分类号： C12Q168

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

公开(公告)号：US06337202B1

公开(公告)日：2002-01-08

申请号：US09511477

申请日：2000-02-23

申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

IPC分类号： C12N924

CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述了含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。