公开(公告)号：US20140295468A1

公开(公告)日：2014-10-02

申请号：US14229634

申请日：2014-03-28

Applicant: FUJIFILM Corporation

Inventor： Noriyuki KASAGI ,  Hiroyuki CHIKU ,  Dai UJIHARA ,  Toshihide YOSHITANI

IPC: G01N33/543

CPC classification number: G01N33/54306 ,  G01N33/54366 ,  G01N33/582 ,  G01N33/585

Abstract: The test substance assay method includes (i) obtaining a mixed solution by mixing (a) first dry particles that are modified with first binding substances exhibiting binding properties specific to a test substance, have an average particle size of 100 nm to 200 nm, and have labels, and (b) second dry particles that are modified with second binding substances not exhibiting binding properties specific to the test substance, have an average particle size of 100 nm to 200 nm, and do not have labels with (c) a test sample solution containing the test substance; (ii) applying the mixed solution onto a substrate; (iii) causing the test substance to be trapped in a reaction site on the substrate that has third binding substances having binding properties specific to the test substance or has substances exhibiting binding properties to the first binding substances; and (iv) detecting the test substance.

Abstract translation: 测试物质测定方法包括：（i）通过混合（a）第一干燥颗粒，所述第一干燥颗粒用具有特定于测试物质的结合特性的第一结合物质进行改性，平均粒径为100nm至200nm，以及 具有标签，并且（b）用不具有特定于测试物质的结合特性的第二结合物修饰的第二干燥颗粒具有100nm至200nm的平均粒度，并且没有标记（c）测试 含有测试物质的样品溶液; （ii）将混合溶液施加到基材上; （iii）使测试物质被捕获在具有与测试物质特异性结合特性的第三结合物质或具有与第一结合物质具有结合性的物质的基材上的反应部位中; 和（iv）检测测试物质。