公开(公告)号：US20240085298A1

公开(公告)日：2024-03-14

申请号：US18518386

申请日：2023-11-22

Applicant: FUJIFILM Corporation

Inventor： Sohichiro NAKAMURA ,  Kenichi Hamada

IPC: G01N15/02

CPC classification number: G01N15/0211

Abstract: Provided are a particle measurement device and a particle measurement method capable of measuring a refractive index or a complex refractive index and a particle size distribution of a single type of particles included in a dispersion liquid. A particle measurement device includes a light source unit that irradiates a dispersion liquid including a single type of particles with measurement light, a parameter setting unit that sets at least one of a scattering angle or a measurement wavelength as a measurement parameter, a scattered light measurement unit that obtains a plurality of pieces of scattering intensity data by measuring a scattering intensity of scattered light emitted from the dispersion liquid by the measurement light a plurality of times while changing a value of the set measurement parameter a plurality of times, and a calculation unit that calculates a refractive index and a particle diameter distribution of the single type of particles by calculating scattering intensity time variation characteristic data and scattering intensity parameter-dependent data from the plurality of pieces of scattering intensity data and fitting the calculated scattering intensity time variation characteristic data and the calculated scattering intensity parameter-dependent data using a theoretical formula or a simulation based on a theory of electromagnetic wave behavior that defines a relationship of the refractive index, a particle diameter, and the scattering intensity.

公开(公告)号：US20230375455A1

公开(公告)日：2023-11-23

申请号：US18361890

申请日：2023-07-30

Applicant: FUJIFILM Corporation

Inventor： Kenichi HAMADA ,  Sohichiro NAKAMURA

IPC: G01N15/02

CPC classification number: G01N15/0211 ,  G01N2015/0222

Abstract: Provided are a dynamic light scattering measurement method and a dynamic light scattering measurement device with which a particle size distribution of each type of particle included in a dispersion liquid including particles is obtained. A dynamic light scattering measurement method for a dispersion liquid including a plurality of types of particles includes a measurement step of measuring a scattering intensity of the dispersion liquid a plurality of times to obtain a plurality of pieces of scattering intensity data while changing a value of any one of at least a scattering angle or a measurement wavelength among measurement parameters, a calculation step of calculating a plurality of pieces of scattering intensity time variation characteristic data and a plurality of scattering intensity parameter-dependent data from the plurality of pieces of scattering intensity data obtained by the measurement step, and a step of obtaining a particle size distribution of each type of particle of a plurality of types of particles by fitting the plurality of pieces of scattering intensity time variation characteristic data and the plurality of pieces of scattering intensity parameter-dependent data, which are obtained by the calculation step, with respect to a theoretical formula that defines a relationship between a particle diameter and the scattering intensity.

公开(公告)号：US20220244243A1

公开(公告)日：2022-08-04

申请号：US17727049

申请日：2022-04-22

Applicant: FUJIFILM Corporation

Inventor： Yuta MURAKAMI ,  Ryusuke OSAKI ,  Sho ONOZAWA ,  Sohichiro NAKAMURA

IPC: G01N33/50 ,  C12N5/074 ,  C12N5/077 ,  G01N15/14

Abstract: There is provided a method including generating a phase contrast image of an aggregate of a pluripotent stem cell from a hologram in which the aggregate is captured; deriving, based on the phase contrast image, an index value indicating a complexity or simplicity of a contour line, the index value being determined according to a relationship between an area of the aggregate and a length of the contour line of the aggregate; and sorting the pluripotent stem cell based on the index value.

公开(公告)号：US20220083004A1

公开(公告)日：2022-03-17

申请号：US17537590

申请日：2021-11-30

Applicant: FUJIFILM Corporation

Inventor： Sohichiro NAKAMURA ,  Sho ONOZAWA ,  Ryusuke OSAKI

IPC: G03H1/22 ,  G06T7/00 ,  G06T5/50

Abstract: An image processing apparatus includes an acquisition unit that acquires a hologram obtained by imaging a plurality of granules contained within an imaging visual field, a generation unit that generates, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured, a specifying unit that specifies a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images, and an extraction unit that extracts the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.

公开(公告)号：US20200342599A1

公开(公告)日：2020-10-29

申请号：US16928974

申请日：2020-07-14

Applicant: FUJIFILM Corporation

Inventor： Sohichiro NAKAMURA ,  Ryusuke OSAKI ,  Sho ONOZAWA ,  Akihiro ASAKURA

IPC: G06T7/00 ,  G06T7/62 ,  G01N21/45 ,  G01N15/06 ,  C12Q1/02

Abstract: Provided is a determination method capable of non-destructively and simply determining a state of a sphere that is an aggregate of a plurality of cells. A phase difference image of a sphere that is an aggregate of a plurality of cells is generated from a hologram obtained by imaging the sphere, and a state of the sphere is determined on the basis of the phase difference image and a shape index value corresponding to a shape of the sphere.