公开(公告)号：US20190136293A1

公开(公告)日：2019-05-09

申请号：US16247323

申请日：2019-01-14

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael R. Reshatoff ,  Edgar O. Ong ,  James J. Hogan

IPC: C12Q1/68 ,  C12Q1/689

CPC classification number: C12Q1/68 ,  C12Q1/689 ,  C12Q2304/00 ,  Y02A50/451

Abstract: Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.

公开(公告)号：US09845509B2

公开(公告)日：2017-12-19

申请号：US14075944

申请日：2013-11-08

Applicant: Gen-Probe Incorporated

Inventor： Jennifer J. Bungo ,  James J. Hogan ,  Reinhold B. Pollner ,  Marie K. Hudspeth ,  Shannon K. Kaplan ,  Elizabeth M. Marlowe

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/686 ,  Y02A50/451

Abstract: Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, and detection probes that hybridize specifically to Legionella pneumophila 23S rRNA sequences or DNA encoding 23S rRNA. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of Legionella pneumophila 23S rRNA sequences or DNA encoding 23S rRNA. Methods are disclosed for amplifying and/or detecting the presence of L. pnuemophila in samples by using the disclosed compositions in in vitro methods that include nucleic acid amplification and/or detection of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of Legionella pneumophila 23S rRNA sequences or DNA encoding 23S rRNA.

公开(公告)号：US09765403B2

公开(公告)日：2017-09-19

申请号：US14938630

申请日：2015-11-11

Applicant: Gen-Probe Incorporated

Inventor： Shannon K. Kaplan ,  Kristin W. Livezey ,  Michael M. Becker ,  James J. Hogan

IPC: C12Q1/68

Abstract: Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.