公开(公告)号：US20150119259A1

公开(公告)日：2015-04-30

申请号：US14391337

申请日：2013-04-08

Applicant: Jingyue JU ,  Randall DAVIS ,  Roger CHEN ,  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  GENIA TECHNOLOGIES, INC.

Inventor： Jingyue Ju ,  Randall Davis ,  Roger Chen

IPC: C12Q1/68

Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

Abstract translation: 本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。 在一些方面，本公开涉及使用标签分子对核酸进行测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

公开(公告)号：US20130244340A1

公开(公告)日：2013-09-19

申请号：US13745688

申请日：2013-01-18

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall Davis ,  Roger Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2565/607

Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

Abstract translation: 本公开提供用于分子鉴定和使用纳米孔的聚合物（例如，核酸）测序的系统和方法。 聚合物可以通过纳米孔或靠近纳米孔，聚合物的各种亚基可能影响流过纳米孔的电流。 可以通过测量跨过纳米孔和/或膜施加的多个电压的电流来识别各种亚基。 在一些情况下，标记的核苷酸的聚合会向纳米孔呈现标签分子，其可以通过测量施加在纳米孔和/或膜上的多个电压的电流来鉴定。 本文还提供了用纳米孔对双链核酸分子的有义链和反义链进行测序的系统和方法，以及使用核糖核酸（RNA）速度突变分子来减缓核酸分子通过的方法或 靠近纳米孔。