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    Nucleic acid sequencing using tags
    1.
    发明授权
    Nucleic acid sequencing using tags 有权

    公开(公告)号：US09605309B2

    公开(公告)日：2017-03-28

    申请号：US14073445

    申请日：2013-11-06

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

    Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

    Methods for creating bilayers for use with nanopore sensors
    4.
    发明授权
    Methods for creating bilayers for use with nanopore sensors 有权

    公开(公告)号：US10316360B2

    公开(公告)日：2019-06-11

    申请号：US15809725

    申请日：2017-11-10

    Applicant: GENIA TECHNOLOGIES, INC.

    Inventor： Randall Davis ,  Roger Chen

    IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

    Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

    Chip set-up and high-accuracy nucleic acid sequencing
    6.
    发明授权
    Chip set-up and high-accuracy nucleic acid sequencing 有权
    芯片设置和高精度核酸测序

    公开(公告)号：US09494554B2

    公开(公告)日：2016-11-15

    申请号：US13918626

    申请日：2013-06-14

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Kevin Deierling

    IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

    CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

    Abstract: The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm2. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.

    Abstract translation: 本公开提供了用于测序核酸分子的装置，系统和方法。 使用包含独立可寻址的纳米孔传感器阵列的芯片，密度为每1mm 2至少约500个位点，核酸分子可以以高精度（例如，单次通过中大于97％）进行测序。 单个纳米孔传感器可以包括邻近或接近检测电极的膜中的纳米孔。

    Process for biosensor well formation
    7.
    发明授权
    Process for biosensor well formation 有权
    生物传感器井形成过程

    公开(公告)号：US09322062B2

    公开(公告)日：2016-04-26

    申请号：US14521427

    申请日：2014-10-22

    Applicant: Genia Technologies, Inc.

    Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech ,  Hui Tian ,  Robert Yuan ,  John Foster ,  Roger Chen

    IPC: G01N27/327 ,  C12Q1/68 ,  C12M1/34

    CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

    Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

    Abstract translation: 公开了用于分子检测和感测的生物芯片。 生物芯片包括基底。 生物芯片包括形成在衬底上的多个离散位置，其密度大于每平方毫米500个孔。 每个离散部位包括设置在基底上以形成孔的侧壁。 每个离散位置包括设置在井的底部的电极。 在一些实施例中，形成井，使得井之间的串扰减少。 在一些实施例中，设置在阱的底部的电极被组织成电极组，其中每组电极共享公共对电极。 在一些实施例中，设置在阱底部的电极具有专用的对电极。 在一些实施例中，侧壁的表面被硅烷化，使得表面有助于在孔中或邻近孔的膜的形成。

    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES
    8.
    发明申请
    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES 审中-公开
    通过纳米检测标签分子的核酸序列

    公开(公告)号：US20150119259A1

    公开(公告)日：2015-04-30

    申请号：US14391337

    申请日：2013-04-08

    Applicant: Jingyue JU ,  Randall DAVIS ,  Roger CHEN ,  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  GENIA TECHNOLOGIES, INC.

    Inventor： Jingyue Ju ,  Randall Davis ,  Roger Chen

    IPC: C12Q1/68

    Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

    Abstract translation: 本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。 在一些方面，本公开涉及使用标签分子对核酸进行测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

    Nanopore Based Molecular Detection and Sequencing
    9.
    发明申请
    Nanopore Based Molecular Detection and Sequencing 审中-公开
    基于纳米孔的分子检测和测序

    公开(公告)号：US20130244340A1

    公开(公告)日：2013-09-19

    申请号：US13745688

    申请日：2013-01-18

    Applicant: GENIA TECHNOLOGIES, INC.

    Inventor： Randall Davis ,  Roger Chen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2565/607

    Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

    Abstract translation: 本公开提供用于分子鉴定和使用纳米孔的聚合物（例如，核酸）测序的系统和方法。 聚合物可以通过纳米孔或靠近纳米孔，聚合物的各种亚基可能影响流过纳米孔的电流。 可以通过测量跨过纳米孔和/或膜施加的多个电压的电流来识别各种亚基。 在一些情况下，标记的核苷酸的聚合会向纳米孔呈现标签分子，其可以通过测量施加在纳米孔和/或膜上的多个电压的电流来鉴定。 本文还提供了用纳米孔对双链核酸分子的有义链和反义链进行测序的系统和方法，以及使用核糖核酸（RNA）速度突变分子来减缓核酸分子通过的方法或 靠近纳米孔。

    Methods for creating bilayers for use with nanopore sensors
    10.
    发明授权
    Methods for creating bilayers for use with nanopore sensors 有权

    公开(公告)号：US11299781B2

    公开(公告)日：2022-04-12

    申请号：US16396284

    申请日：2019-04-26

    Applicant: GENIA TECHNOLOGIES, INC.

    Inventor： Randall Davis ,  Roger Chen

    IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

    Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

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