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    Nucleic acid sequencing using tags
    3.
    发明授权
    Nucleic acid sequencing using tags 有权

    公开(公告)号：US09605309B2

    公开(公告)日：2017-03-28

    申请号：US14073445

    申请日：2013-11-06

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

    Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

    High Speed Molecular Sensing with Nanopores
    4.
    发明申请
    High Speed Molecular Sensing with Nanopores 有权
    高分子感应纳米孔

    公开(公告)号：US20150111779A1

    公开(公告)日：2015-04-23

    申请号：US14521469

    申请日：2014-10-23

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis

    IPC: C12Q1/68

    Abstract: Described herein are methods and devices for capturing and determining the identity of molecules using nanopores. The molecules can be counted, sorted and/or binned rapidly in a parallel manner using a large number of nanopores (e.g., 132,000 nanopores reading 180 million molecules in 1 hour). This fast capture and reading of a molecule can be used to capture probe molecules or other molecules that have been generated to represent an original, hard to detect molecule or portions of an original molecule. Precise counting of sample molecules or surrogates for sample molecules can occur. The methods and devices described herein can, among other things, replace flow cytometers and other counting instruments (e.g., while providing increased precision and throughput relative to a flow cytometer). In some cases, the devices and methods capture and hold particular molecules or surrogates of molecules in the nanopores and then eject them into clean solution to perform a capture, sorting, and binning function similar to flow cytometers.

    Abstract translation: 本文描述的是使用纳米孔捕获和确定分子的身份的方法和装置。 可以使用大量的纳米孔（例如，在1小时内读取1.8亿分子的132,000个纳米孔）以平行方式快速计数，分选和/或分箱分子。 分子的这种快速捕获和读取可用于捕获已经产生的探针分子或其它分子，以代表原始的，难以检测的分子或原始分子的部分。 可能会发生样品分子或样品分子替代品的精确计数。 除此之外，本文所述的方法和装置可以替代流式细胞仪和其他计数仪器（例如，相对于流式细胞仪提供更高的精度和通量）。 在一些情况下，装置和方法捕获并保持纳米孔中的特定分子或分子的替代物，然后将其喷射到干净的溶液中以执行与流式细胞仪相似的捕获，分选和合并功能。

    Methods for creating bilayers for use with nanopore sensors
    5.
    发明授权
    Methods for creating bilayers for use with nanopore sensors 有权

    公开(公告)号：US11299781B2

    公开(公告)日：2022-04-12

    申请号：US16396284

    申请日：2019-04-26

    Applicant: GENIA TECHNOLOGIES, INC.

    Inventor： Randall Davis ,  Roger Chen

    IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

    Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

    NUCLEIC ACID SEQUENCING USING TAGS
    6.
    发明申请
    NUCLEIC ACID SEQUENCING USING TAGS 有权
    使用标签的核酸序列测序

    公开(公告)号：US20140134616A1

    公开(公告)日：2014-05-15

    申请号：US14073445

    申请日：2013-11-06

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum ,  Michael Dorwart

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

    Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

    Abstract translation: 本公开提供了用于对核酸样品进行测序的芯片，系统和方法。 将标记的核苷酸提供到包含膜中的纳米孔的反应室中。 标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，该标签借助于纳米孔可检测。 接下来，标记的核苷酸的单个标记的核苷酸可以掺入与衍生自核酸样品的单链核酸分子互补的生长链中。 借助纳米孔，可以在掺入单个标记的核苷酸时检测与单个标记的核苷酸相关的标签。 当标签从核苷酸释放时，可以借助纳米孔检测标签。

    High speed molecular sensing with nanopores
    7.
    发明授权
    High speed molecular sensing with nanopores 有权

    公开(公告)号：US10421995B2

    公开(公告)日：2019-09-24

    申请号：US14521469

    申请日：2014-10-23

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis

    IPC: C12Q1/68 ,  C12Q1/00 ,  C12M1/34 ,  C12Q1/6869 ,  C12Q1/6816

    Abstract: Described herein are methods and devices for capturing and determining the identity of molecules using nanopores. The molecules can be counted, sorted and/or binned rapidly in a parallel manner using a large number of nanopores (e.g., 132,000 nanopores reading 180 million molecules in 1 hour). This fast capture and reading of a molecule can be used to capture probe molecules or other molecules that have been generated to represent an original, hard to detect molecule or portions of an original molecule. Precise counting of sample molecules or surrogates for sample molecules can occur. The methods and devices described herein can, among other things, replace flow cytometers and other counting instruments (e.g., while providing increased precision and throughput relative to a flow cytometer). In some cases, the devices and methods capture and hold particular molecules or surrogates of molecules in the nanopores and then eject them into clean solution to perform a capture, sorting, and binning function similar to flow cytometers.

    Methods for creating bilayers for use with nanopore sensors
    8.
    发明授权
    Methods for creating bilayers for use with nanopore sensors 有权

    公开(公告)号：US10316360B2

    公开(公告)日：2019-06-11

    申请号：US15809725

    申请日：2017-11-10

    Applicant: GENIA TECHNOLOGIES, INC.

    Inventor： Randall Davis ,  Roger Chen

    IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

    Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

    Chip set-up and high-accuracy nucleic acid sequencing
    10.
    发明授权
    Chip set-up and high-accuracy nucleic acid sequencing 有权
    芯片设置和高精度核酸测序

    公开(公告)号：US09494554B2

    公开(公告)日：2016-11-15

    申请号：US13918626

    申请日：2013-06-14

    Applicant: Genia Technologies, Inc.

    Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Kevin Deierling

    IPC: G01N27/447 ,  G01N33/487 ,  C12Q1/68

    CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

    Abstract: The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm2. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.

    Abstract translation: 本公开提供了用于测序核酸分子的装置，系统和方法。 使用包含独立可寻址的纳米孔传感器阵列的芯片，密度为每1mm 2至少约500个位点，核酸分子可以以高精度（例如，单次通过中大于97％）进行测序。 单个纳米孔传感器可以包括邻近或接近检测电极的膜中的纳米孔。

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