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公开(公告)号：US20110023352A1
公开(公告)日：2011-02-03
申请号：US12810142
申请日：2007-12-27
IPC分类号： C10L1/188
CPC分类号： C10L1/026 , C10G2300/1011 , Y02E50/13 , Y02P30/20 , Y02T50/678
摘要： Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.
摘要翻译： 本文提供的是包含超过50％的中链脂肪酸或其脂肪酸烷基酯并具有低熔点的混合油或脂肪酸。 这种共混物可用作燃料或作为用于生产例如生物柴油的原料。 还提供遗传改变或修饰的植物，被修饰，使得植物产生的中链脂肪酸的量增加。 进一步提供了一种预测脂肪酸甲酯共混物的熔点的方法，以及用于鉴定适合用作例如生物柴油的共混物的方法。
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公开(公告)号：US20090298150A1
公开(公告)日：2009-12-03
申请号：US12471273
申请日：2009-05-22
申请人： Keith A. Walker , Mark E. Knuth , Noel M. Fong , Peter R. Beetham
发明人： Keith A. Walker , Mark E. Knuth , Noel M. Fong , Peter R. Beetham
CPC分类号： C12P5/007
摘要： Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically altered yeast and uses therefore. In some aspects and embodiments, the genetically altered yeast produce isoprenoids, preferably squalene. The genetically altered yeast may have alterations in the expression or activity of enzymes involved in squalene production. for example, acetyl-CoA carboxylase (or “ACCase”), HMG-CoA reductase, squalene epoxidase, and squalene synthase. One or more genes of a genetically altered yeast may be modified by gene repair oligonucleobases. Also are provided methods of producing squalene using a genetically altered yeast. The invention also provides squalene produced by genetically altered yeast.
摘要翻译： 提供了用于产生类异戊二烯（包括角鲨烯）的组合物和方法。 在某些方面和提供的实施方案是经遗传改变的酵母并因此使用。 在一些方面和实施方案中，遗传改变的酵母产生类异戊二烯，优选角鲨烯。 遗传改变的酵母可能在参与角鲨烯生产的酶的表达或活性方面具有改变。 例如乙酰辅酶A羧化酶（或“ACCase”），HMG-CoA还原酶，角鲨烯环氧酶和角鲨烯合酶。 遗传改变的酵母的一个或多个基因可以通过基因修复寡核苷酸修饰。 还提供了使用遗传改变的酵母生产角鲨烯的方法。 本发明还提供由遗传改变的酵母产生的角鲨烯。
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公开(公告)号：US20120122223A1
公开(公告)日：2012-05-17
申请号：US13247954
申请日：2011-09-28
申请人： Gregory F.W. Gocal , Peter R. Beetham , Aura de Schopke , Sarah Dumm , James Pearce , Christian Schopke , Keith A. Walker
发明人： Gregory F.W. Gocal , Peter R. Beetham , Aura de Schopke , Sarah Dumm , James Pearce , Christian Schopke , Keith A. Walker
IPC分类号： C12N5/04
CPC分类号： C12N15/8274 , C12N9/001
摘要： Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant.
摘要翻译： 提供与转基因植物或非转基因植物中的基因和/或蛋白质突变相关的组合物和方法。 在某些实施方案中，本公开涉及原卟啉原IX（PPX）基因中的突变。 在一些实施方案中，本公开涉及除草剂抗性的植物。
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公开(公告)号：US08106259B2
公开(公告)日：2012-01-31
申请号：US12463891
申请日：2009-05-11
IPC分类号： C12N15/82
CPC分类号： C12N15/8245 , C12N15/102 , C12N15/8206 , C12N15/8213 , C12N15/8246 , C12N15/8247 , C12N15/8249 , C12N15/8251 , C12N15/8255 , C12N15/8274 , C12N15/8289
摘要： Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores.
摘要翻译： 混合双链寡核苷酸（MDON）用于影响植物目标DNA序列中的位点特异性遗传改变。 通过电穿孔将MDON引入小孢子中。 此后，通过发芽小孢子产生具有所需遗传改变的植物。
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公开(公告)号：US08029579B2
公开(公告)日：2011-10-04
申请号：US12306477
申请日：2007-06-27
IPC分类号： C10L1/18
CPC分类号： C10L1/026 , A23D9/00 , C10L2200/0476 , C11C1/08 , C11C3/003 , C12N9/0083 , C12N15/8247 , Y02E50/13
摘要： Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.
摘要翻译： 本文提供的是包含超过50％的中链脂肪酸或其脂肪酸烷基酯并具有低熔点的混合油或脂肪酸。 这种共混物可用作燃料或作为用于生产例如生物柴油的原料。 还提供遗传改变或修饰的植物，被修饰，使得植物产生的中链脂肪酸的量增加。 进一步提供了一种预测脂肪酸甲酯共混物的熔点的方法，以及用于鉴定适合用作例如生物柴油的共混物的方法。
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公开(公告)号：US10035991B2
公开(公告)日：2018-07-31
申请号：US11941666
申请日：2007-11-16
CPC分类号： C12N9/1092 , A01H1/06 , A01H5/10 , C12N15/8213 , C12N15/8275
摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.
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公开(公告)号：US20170051219A1
公开(公告)日：2017-02-23
申请号：US13752330
申请日：2013-01-28
申请人： Mark Knuth , Peter R. Beetham , Keith Walker , Greg F.W. Gocal
发明人： Mark Knuth , Peter R. Beetham , Keith Walker , Greg F.W. Gocal
CPC分类号： C10L1/026 , A23D9/00 , C10L2200/0476 , C11C1/08 , C11C3/003 , C12N9/0083 , C12N15/8247 , Y02E50/13
摘要： Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.
摘要翻译： 本文提供的是包含超过50％的中链脂肪酸或其脂肪酸烷基酯并具有低熔点的混合油或脂肪酸。 这种共混物可用作燃料或作为用于生产例如生物柴油的原料。 还提供遗传改变或修饰的植物，被修饰，使得植物产生的中链脂肪酸的量增加。 进一步提供了一种预测脂肪酸甲酯共混物的熔点的方法，以及用于鉴定适合用作例如生物柴油的共混物的方法。
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公开(公告)号：US06870075B1
公开(公告)日：2005-03-22
申请号：US09685403
申请日：2000-10-10
CPC分类号： C12N9/1092 , A01H1/06 , A01H5/10 , C12N15/8213 , C12N15/8275
摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide.
摘要翻译： 本发明涉及对膦酰甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的非转基因植物的生产。 本发明还涉及重组型寡核苷酸在编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）的基因中的植物的染色体或附加型序列中进行所需的突变的用途。 基本上保持野生型蛋白质的催化活性的突变蛋白质允许植物对膦酰基甲基甘氨酸家族的除草剂的抗性或耐受性增加，并允许植物，其器官的基本上正常的生长或发育， 组织或细胞，与野生型植物相比，无论是否存在除草剂。
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