公开(公告)号：US20190292568A1

公开(公告)日：2019-09-26

申请号：US16358461

申请日：2019-03-19

Applicant: Inscripta, Inc.

Inventor： Christopher Abraham ,  Charles Johnson ,  Clint Davis ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.