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公开(公告)号：US20220389461A1
公开(公告)日：2022-12-08
申请号：US17752122
申请日：2022-05-24
Applicant: Inscripta, Inc.
Inventor： Randy Lacey , Bryan Leland , Eileen Spindler , Patrick Westfall , Skylar Stefani , Catherine Baranowski
Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, methods, instruments, systems, and nucleic acids synthetic cassettes that improve the efficiency of gene editing using CRISPR enzymes in diploid cells—either on both chromosomes or selectively in one chromosome—without incurring loss of heterozygosity (LOH) and its often deleterious effects.
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公开(公告)号：US11254942B2
公开(公告)日：2022-02-22
申请号：US17353282
申请日：2021-06-21
Applicant: Inscripta, Inc.
Inventor： Tian Tian , Charles Johnson , Eileen Spindler
IPC: C12N15/70 , C12N15/113 , C12N9/22
Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.
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公开(公告)号：US20210317464A1
公开(公告)日：2021-10-14
申请号：US17355081
申请日：2021-06-22
Applicant: Inscripta, Inc.
Inventor： Krishna Kannan , Miles Gander , Eileen Spindler , Paul Hardenbol
Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
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公开(公告)号：US11891609B2
公开(公告)日：2024-02-06
申请号：US17680289
申请日：2022-02-25
Applicant: Inscripta, Inc.
Inventor： Tian Tian , Eileen Spindler , Charles Johnson , Clint Davis
IPC: C12N15/74 , C12N15/113 , C12N1/20 , C12N9/22
CPC classification number: C12N15/74 , C12N1/20 , C12N9/22 , C12N15/113 , C12N2310/20
Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.
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公开(公告)号：US11685889B2
公开(公告)日：2023-06-27
申请号：US17734037
申请日：2022-04-30
Applicant: Inscripta, Inc.
Inventor： Andrew Garst , Richard Fox , Eileen Spindler , Amy Hiddessen , Phillip Belgrader , Don Masquelier , Bruce Chabansky , Michael Graige
CPC classification number: C12M35/08 , C12M23/44 , C12M33/14 , C12N15/11 , C12N15/902 , C12N2310/20
Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.
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Patent Agency Ranking
公开(公告)号：US20220177899A1
公开(公告)日：2022-06-09
申请号：US17680289
申请日：2022-02-25
Applicant: Inscripta, Inc.
Inventor： Tian Tian , Eileen Spindler , Charles Johnson , Clint Davis
IPC: C12N15/74 , C12N15/113 , C12N1/20 , C12N9/22
Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome
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公开(公告)号：US20210207149A1
公开(公告)日：2021-07-08
申请号：US17199413
申请日：2021-03-11
Applicant: Inscripta, Inc.
Inventor： Miles Gander , Tian Tian , Eileen Spindler
Abstract: The present disclosure provides compositions and methods to increase the percentage of edited yeast cells in a cell population when employing nucleic acid-guided editing, and automated multi-module instruments for performing these methods.
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公开(公告)号：US20210130834A1
公开(公告)日：2021-05-06
申请号：US17140056
申请日：2021-01-02
Applicant: Inscripta, Inc.
Inventor： Krishna Kannan , Miles Gander , Eileen Spindler , Paul Hardenbol
Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
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公开(公告)号：US11268061B2
公开(公告)日：2022-03-08
申请号：US17401362
申请日：2021-08-13
Applicant: Inscripta, Inc.
Inventor： Andrew Garst , Michael Graige , Richard Fox , Eileen Spindler , Amy Hiddessen , Phillip Belgrader , Don Masquelier , Bruce Chabansky
Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.
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公开(公告)号：US20220025359A1
公开(公告)日：2022-01-27
申请号：US17300518
申请日：2021-07-27
Applicant: Inscripta, Inc.
Inventor： Tian Tian , Eileen Spindler , Clint Davis , Charles Johnson , Andrew Garst
Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.
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