公开(公告)号：US10508273B2

公开(公告)日：2019-12-17

申请号：US16392625

申请日：2019-04-24

Applicant: Inscripta, Inc.

Inventor： Deanna Church ,  Stephen Federowicz ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81 ,  C40B50/04 ,  C40B40/02

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20220195625A1

公开(公告)日：2022-06-23

申请号：US17690641

申请日：2022-03-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20210002628A1

公开(公告)日：2021-01-07

申请号：US16988696

申请日：2020-08-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US10774446B1

公开(公告)日：2020-09-15

申请号：US16904640

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C40B50/06 ,  C40B70/00 ,  C40B40/10 ,  C12N15/90 ,  C12N15/85 ,  C40B20/04 ,  C40B30/04 ,  C12N9/22 ,  C07K16/28 ,  C40B40/02 ,  C07K14/74 ,  C12N15/81

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US10774324B2

公开(公告)日：2020-09-15

申请号：US16670396

申请日：2019-10-31

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81 ,  C40B50/04 ,  C40B40/02

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20200216977A1

公开(公告)日：2020-07-09

申请号：US16827164

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US11555184B2

公开(公告)日：2023-01-17

申请号：US16988696

申请日：2020-08-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/19 ,  C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81 ,  C40B50/04 ,  C40B40/02

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US11293117B2

公开(公告)日：2022-04-05

申请号：US17016454

申请日：2020-09-10

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C07K14/74 ,  C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20210381007A1

公开(公告)日：2021-12-09

申请号：US17338603

申请日：2021-06-03

Applicant: Inscripta, Inc.

Inventor： Janine Mok ,  Miles Gander ,  Andrew Garst ,  Burak Dura ,  Deanna Church

IPC: C12N15/90 ,  C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods and modules to edit live cells and to subsequently correlate the resulting cellular nucleic acids of the edited cells to the edits.

公开(公告)号：US20200318256A1

公开(公告)日：2020-10-08

申请号：US16904640

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C40B70/00 ,  C40B40/10 ,  C12N15/90 ,  C12N15/85 ,  C40B20/04 ,  C12N15/81 ,  C12N15/10 ,  C12N9/22 ,  C07K16/28 ,  C40B40/02 ,  C07K14/74 ,  C40B30/04

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.