公开(公告)号：US11697826B2

公开(公告)日：2023-07-11

申请号：US16421783

申请日：2019-05-24

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113 ,  C07K14/195

CPC classification number: C12N15/902 ,  C07K14/195 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C07K2319/85 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20230174961A1

公开(公告)日：2023-06-08

申请号：US18101543

申请日：2023-01-25

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Christian Siltanen

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, the disclosure provides nucleic acid cassettes, plasmids, vectors, and compositions comprising the same that employ homologous recombination for genome engineering by having a CRISPR nuclease cause a specific DSB while tethered to a repair nucleic acid.

公开(公告)号：US11584928B2

公开(公告)日：2023-02-21

申请号：US16295393

申请日：2019-03-07

Applicant: The Regents of the University of Colorado ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20230049357A1

公开(公告)日：2023-02-16

申请号：US17969715

申请日：2022-10-20

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US20220112508A1

公开(公告)日：2022-04-14

申请号：US17555395

申请日：2021-12-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12N15/68 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20220112452A1

公开(公告)日：2022-04-14

申请号：US17555336

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20210388391A1

公开(公告)日：2021-12-16

申请号：US17387860

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20210371799A1

公开(公告)日：2021-12-02

申请号：US17401362

申请日：2021-08-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

公开(公告)号：US20210317400A1

公开(公告)日：2021-10-14

申请号：US17355901

申请日：2021-06-23

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US11142740B2

公开(公告)日：2021-10-12

申请号：US16536049

申请日：2019-08-08

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Michael Graige ,  Richard Fox ,  Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Don Masquelier ,  Bruce Chabansky

IPC: C12M3/00 ,  C12N15/00 ,  B01L3/00 ,  C12M1/42 ,  C12N15/11 ,  C12N15/90 ,  C12M1/26

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.