公开(公告)号：US20190323147A1

公开(公告)日：2019-10-24

申请号：US16392605

申请日：2019-04-23

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C07K16/28 ,  C40B40/10 ,  C40B30/04

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20220267747A1

公开(公告)日：2022-08-25

申请号：US17741326

申请日：2022-05-10

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220243361A1

公开(公告)日：2022-08-04

申请号：US17728135

申请日：2022-04-25

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N15/90 ,  C12N15/10 ,  C40B40/10 ,  C12N15/85 ,  C07K16/28 ,  C40B30/04 ,  C40B40/02 ,  C07K14/74 ,  C12N15/81 ,  C12N9/22 ,  C40B20/04 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20220177865A1

公开(公告)日：2022-06-09

申请号：US17680279

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20220073892A1

公开(公告)日：2022-03-10

申请号：US17526951

申请日：2021-11-15

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US10711374B1

公开(公告)日：2020-07-14

申请号：US16827164

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C07K14/70 ,  C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US10526598B2

公开(公告)日：2020-01-07

申请号：US16392611

申请日：2019-04-23

Applicant: Inscripta, Inc.

Inventor： Deanna Church ,  Stephen Federowicz ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N15/81 ,  C07K14/74 ,  C40B70/00 ,  C40B20/04 ,  C40B40/02 ,  C12N15/85

Abstract: The present disclosure automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptide antigens to identify potential TCR binding targets. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20190323000A1

公开(公告)日：2019-10-24

申请号：US16392625

申请日：2019-04-24

Applicant: Inscripta, Inc.

Inventor： Deanna Church ,  Stephen Federowicz ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20220195625A1

公开(公告)日：2022-06-23

申请号：US17690641

申请日：2022-03-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US11008557B1

公开(公告)日：2021-05-18

申请号：US17123067

申请日：2020-12-15

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.