公开(公告)号：US20110053189A1

公开(公告)日：2011-03-03

申请号：US12658268

申请日：2010-02-09

申请人： John G. Hoey ,  Denise P. Dimitrov ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Denise P. Dimitrov ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： G01N33/53 ,  C12P21/06 ,  C12N1/21 ,  C12N15/63 ,  C07K14/29 ,  C07H21/04

CPC分类号： C07K14/195 ,  G01N33/6854 ,  G01N2469/20

摘要： Disclosed are two (2) proteins in the Type IV Secretion System (TIVSS) in Anaplasma phagocytophilum (namely, virB 10 and virB11) useful in the ELISA detection of Anaplasma pathogen. The recombinant expression of virB 10 and virB 11 and their use as kits for ELISA are also disclosed.

摘要翻译： 公开了在Anaplasma phagocytophilum中的IV型分泌系统（TIVSS）中的两种（2）蛋白质（即，virB10和virB11），其可用于ELISA检测Anaplasma病原体。 还公开了virB10和virB11的重组表达及其作为ELISA试剂盒的用途。

公开(公告)号：US20130040323A1

公开(公告)日：2013-02-14

申请号：US13588134

申请日：2012-08-17

申请人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： G01N33/566

CPC分类号： C07K14/195 ,  G01N33/56911 ,  G01N33/6893 ,  G01N2469/20

摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

摘要翻译： 披露了巴氏杆菌的新型抗原的克隆和表达。 发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。 本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。 还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

公开(公告)号：US08268569B2

公开(公告)日：2012-09-18

申请号：US12462647

申请日：2009-08-06

申请人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： G01N33/53

CPC分类号： C07K14/195 ,  G01N33/56911 ,  G01N33/6893 ,  G01N2469/20

摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

摘要翻译： 披露了巴氏杆菌的新型抗原的克隆和表达。 发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。 本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。 还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

公开(公告)号：US20100209936A1

公开(公告)日：2010-08-19

申请号：US12462647

申请日：2009-08-06

申请人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： G01N33/53 ,  C07K14/195 ,  C07K17/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/02

CPC分类号： C07K14/195 ,  G01N33/56911 ,  G01N33/6893 ,  G01N2469/20

摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

摘要翻译： 披露了巴氏杆菌的新型抗原的克隆和表达。 发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。 本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。 还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

公开(公告)号：US08580271B2

公开(公告)日：2013-11-12

申请号：US13588134

申请日：2012-08-17

申请人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： A61K39/02

CPC分类号： C07K14/195 ,  G01N33/56911 ,  G01N33/6893 ,  G01N2469/20

摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

摘要翻译： 披露了巴氏杆菌的新型抗原的克隆和表达。 发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。 本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。 还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

公开(公告)号：US20140141457A1

公开(公告)日：2014-05-22

申请号：US14048548

申请日：2013-10-08

申请人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

发明人： John G. Hoey ,  Lisa P. Huang ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： C07K14/195 ,  G01N33/68

CPC分类号： C07K14/195 ,  G01N33/56911 ,  G01N33/6893 ,  G01N2469/20

摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

摘要翻译： 披露了巴氏杆菌的新型抗原的克隆和表达。 发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。 本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。 还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

公开(公告)号：US08283439B2

公开(公告)日：2012-10-09

申请号：US12462602

申请日：2009-08-06

申请人： Lisa P. Huang ,  John G. Hoey ,  Martin E. Adelson ,  Eli Mordechai

发明人： Lisa P. Huang ,  John G. Hoey ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： C07K14/195 ,  G01N33/53

CPC分类号： G01N33/56911 ,  C07K14/195 ,  G01N33/6854

摘要： The present disclosure describes recombinant and synthetic polypeptides of Bartonella henselae and related methods and kits for the detection of antibodies specific for Bartonella henselae. The 17-kDa polypeptides, methods and kits are useful in the detection of recent and/or ongoing infections with Bartonella henselae, which can be useful in the diagnosis of Cat Scratch Disease (CSD).

摘要翻译： 本公开描述了贝氏杆菌的重组和合成多肽以及用于检测针对巴氏杆菌的抗体的相关方法和试剂盒。 17-kDa多肽，方法和试剂盒可用于检测近来和/或持续感染巴氏杆菌，其可用于猫抓痕病（CSD）的诊断。

公开(公告)号：US20120058495A1

公开(公告)日：2012-03-08

申请号：US12462602

申请日：2009-08-06

申请人： Lisa P. Huang ,  John G. Hoey ,  Martin E. Adelson ,  Eli Mordechai

发明人： Lisa P. Huang ,  John G. Hoey ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： G01N33/569 ,  C12P21/02 ,  C12N15/70 ,  C12N1/21 ,  C07K7/08 ,  C07K14/195

CPC分类号： G01N33/56911 ,  C07K14/195 ,  G01N33/6854

摘要： The present disclosure describes recombinant and synthetic polypeptides of Bartonella henselae and related methods and kits for the detection of antibodies specific for Bartonella henselae. The 17-kDa polypeptides, methods and kits are useful in the detection of recent and/or ongoing infections with Bartonella henselae, which can be useful in the diagnosis of Cat Scratch Disease (CSD).

公开(公告)号：US08900813B2

公开(公告)日：2014-12-02

申请号：US13199019

申请日：2011-08-17

申请人： Lisa P. Huang

发明人： Lisa P. Huang

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N33/574

CPC分类号： G01N33/57411 ,  C12Q1/6886 ,  C12Q2600/158

摘要： The present invention provides methods and compositions for detecting cervical cancer in a human. The present invention provides CIP2A as a biomarker detectable in a biological sample from cervix of an individual, an increased expression of same is an indication of cervical cancer in the individual. CIP2A mRNA or protein can both serve as reliable biomarkers. In one embodiment, the present invention provides a combined use of CIP2A with at least one additional biomarker selected from the group consisting of Ki67, TOP2A, MCM2, MCM5, p14ARF and p16INK4a, to better serve as a detection means for cervical cancer. The present invention provides a reliable and accurate assay methods and compositions which may be used for detecting cervical cancer in an individual with a high sensitivity and specificity. The present assay provides a novel use of CIP2A as a tool for early detection of cervical cancer in humans.

摘要翻译： 本发明提供了用于检测人类宫颈癌的方法和组合物。 本发明提供CIP2A作为在个体宫颈生物样品中可检测的生物标志物，其增加的表达是个体中子宫颈癌的指征。 CIP2A mRNA或蛋白质均可作为可靠的生物标志物。 在一个实施方案中，本发明提供CIP2A与至少一种选自Ki67，TOP2A，MCM2，MCM5，p14ARF和p16INK4a的另外的生物标志物的组合使用，以更好地用作子宫颈癌的检测手段。 本发明提供了可用于检测具有高灵敏度和特异性的个体中的子宫颈癌的可靠和精确的测定方法和组合物。 本测定提供了CIP2A作为人类早期检测子宫颈癌的工具的新用途。

公开(公告)号：US20120070837A1

公开(公告)日：2012-03-22

申请号：US13199019

申请日：2011-08-17

申请人： Lisa P. Huang ,  Jason Trama

发明人： Lisa P. Huang ,  Jason Trama

IPC分类号： C12Q1/68 ,  G01N33/566

CPC分类号： G01N33/57411 ,  C12Q1/6886 ,  C12Q2600/158

摘要： The present invention provides methods and compositions for detecting cervical cancer in a human. The present invention provides CIP2A as a biomarker detectable in a biological sample from cervix of an individual, an increased expression of same is an indication of cervical cancer in the individual. CIP2A mRNA or protein can both serve as reliable biomarkers. In one embodiment, the present invention provides a combined use of CIP2A with at least one additional biomarker selected from the group consisting of Ki67, TOP2A, MCM2, MCM5, p14ARF and p16INK4a, to better serve as a detection means for cervical cancer. The present invention provides a reliable and accurate assay methods and compositions which may be used for detecting cervical cancer in an individual with a high sensitivity and specificity. The present assay provides a novel use of CIP2A as a tool for early detection of cervical cancer in humans.

摘要翻译： 本发明提供了用于检测人类宫颈癌的方法和组合物。 本发明提供CIP2A作为在个体宫颈生物样品中可检测的生物标志物，其增加的表达是个体中子宫颈癌的指征。 CIP2A mRNA或蛋白质均可作为可靠的生物标志物。 在一个实施方案中，本发明提供CIP2A与至少一种选自Ki67，TOP2A，MCM2，MCM5，p14ARF和p16INK4a的另外的生物标志物的组合使用，以更好地用作子宫颈癌的检测手段。 本发明提供了可用于检测具有高灵敏度和特异性的个体中的子宫颈癌的可靠和精确的测定方法和组合物。 本测定提供了CIP2A作为人类早期检测子宫颈癌的工具的新用途。