公开(公告)号：US20060030022A1

公开(公告)日：2006-02-09

申请号：US11058926

申请日：2005-02-16

Applicant: Jonathan Beckwith ,  Fredrik Aslund ,  Paul Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Lim

Inventor： Jonathan Beckwith ,  Fredrik Aslund ,  Paul Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Lim

IPC: C12N1/21 ,  C12N15/74

CPC classification number: C12N9/0004 ,  C12P21/02

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。