公开(公告)号：US20060030022A1

公开(公告)日：2006-02-09

申请号：US11058926

申请日：2005-02-16

Applicant: Jonathan Beckwith ,  Fredrik Aslund ,  Paul Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Lim

Inventor： Jonathan Beckwith ,  Fredrik Aslund ,  Paul Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Lim

IPC: C12N1/21 ,  C12N15/74

CPC classification number: C12N9/0004 ,  C12P21/02

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。

公开(公告)号：US07410788B2

公开(公告)日：2008-08-12

申请号：US11058926

申请日：2005-02-16

Applicant: Jonathan Beckwith ,  Fredrik Aslund ,  Paul H. Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Eun-ah Lim

Inventor： Jonathan Beckwith ,  Fredrik Aslund ,  Paul H. Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Eun-ah Lim

IPC: C12N1/21 ,  C12N7/00 ,  C12P21/00 ,  C12N15/63

CPC classification number: C12N9/0004 ,  C12P21/02

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。

公开(公告)号：US20060275905A1

公开(公告)日：2006-12-07

申请号：US11189065

申请日：2005-07-25

Applicant: Taeok Bae ,  Fredrik Aslund ,  Olaf Schneewind ,  Dominique Missiakas

Inventor： Taeok Bae ,  Fredrik Aslund ,  Olaf Schneewind ,  Dominique Missiakas

IPC: A61K49/00 ,  C12N15/74 ,  C12N1/21

CPC classification number: A61K49/00 ,  C12N1/20 ,  C12N9/22 ,  C12N15/74

Abstract: The present invention concerns gram positive bacterial mutants, particularly staphylococcus mutants of the Newman strain. It also involves transposable elements that can be used to generate these mutants, as well as methods of creating such mutants with these elements. The inventions also includes methods of using the bacterial mutants to screen for different types of genes, including virulence genes, which can lead to assays for drug compounds useful in the treatment of bacterial infection.

Abstract translation: 本发明涉及革兰氏阳性细菌突变体，特别是Newman菌株的葡萄球菌突变体。 它还涉及可用于产生这些突变体的转座元件，以及用这些元件产生这种突变体的方法。 本发明还包括使用细菌突变体筛选不同类型的基因（包括毒力基因）的方法，其可以导致用于治疗细菌感染的药物化合物的测定。

公开(公告)号：US06872563B1

公开(公告)日：2005-03-29

申请号：US09679705

申请日：2000-10-05

Applicant: Jonathan Beckwith ,  Fredrik Aslund ,  Paul H. Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Eun-ah Lim

Inventor： Jonathan Beckwith ,  Fredrik Aslund ,  Paul H. Bessette ,  George Georgiou ,  Daniel Ritz ,  Jackie Eun-ah Lim

IPC: C12N9/02 ,  C12P21/02 ,  C12N1/20

CPC classification number: C12N9/0004 ,  C12P21/02

Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.

Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。