公开(公告)号：US20060246479A1

公开(公告)日：2006-11-02

申请号：US11351709

申请日：2006-02-10

Applicant: Richard Ebright

Inventor： Richard Ebright

IPC: C12Q1/68 ,  C12N9/22

CPC classification number: G01N33/566 ,  C07K2299/00 ,  C12N9/1247 ,  C12Q1/48 ,  G01N33/569 ,  G01N33/573 ,  G01N2333/9125 ,  G01N2500/00

Abstract: The invention provides a target and methods for specific binding and inhibition of RNA polymerase from bacterial species. The invention provides methods for identifying agents that bind to a bacterial RNA polymerase, and that inhibit an activity of a bacterial RNA polymerase, through interactions with a bacterial RNA polymerase homologous switch-region amino-acid sequence. Said methods comprise preparing a reaction solution comprising the compound to be tested and an entity containing a bacterial RNAP homologous switch-region amino-acid sequence, and detecting binding or inhibition. The invention has applications in control of bacterial gene expression, control of bacterial viability, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.

Abstract translation: 本发明提供了从细菌种类特异性结合和抑制RNA聚合酶的靶标和方法。 本发明提供了通过与细菌RNA聚合酶同源转换区氨基酸序列的相互作用来鉴定结合细菌RNA聚合酶并且抑制细菌RNA聚合酶活性的试剂的方法。 所述方法包括制备包含待测试化合物和含有细菌RNAP同源转换区氨基酸序列的实体的反应溶液，并检测结合或抑制。 本发明可用于控制细菌基因表达，控制细菌活力，控制细菌生长，抗菌化学和抗菌治疗。

公开(公告)号：US20060141531A1

公开(公告)日：2006-06-29

申请号：US11257292

申请日：2005-10-24

Applicant: Richard Ebright ,  Yon Ebright

Inventor： Richard Ebright ,  Yon Ebright

IPC: G01N33/53 ,  C07K16/46 ,  C07K14/47

CPC classification number: C07K7/08 ,  C07K5/0821 ,  C07K5/0827 ,  C07K5/1013 ,  C07K7/06 ,  C07K14/001 ,  G01N33/542 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/805

Abstract: A self-assembled relay probe for detecting a target material is provided including: a first peptide tag bound to the target material; and a first fluorescent conjugate including a first fluorochrome and a first tag binding group; wherein the first fluorescent conjugate selectively associates with the first tag. The probe further includes a second peptide tag bound to the target material; and a second fluorescent conjugate including a second fluorochrome having a longer wavelength and distinct excitation and emission maxima from the first fluorochrome and a second tag binding group. Upon exposure to the target material, the first and second fluorescent conjugates independently associate with the first and second peptide tags, respectively, so as to be a distance apart represented by about 0.1 times R0 to about 2 times R0, such that upon excitation of the first fluorescent conjugate, fluorescence resonance energy transfer results in excitation of the second fluorescent conjugate, yielding detectable emission from the second fluorescent conjugate.

Abstract translation: 提供了一种用于检测靶材料的自组装继电器探针，包括：与目标材料结合的第一肽标签; 和包含第一荧光染料和第一标签结合基团的第一荧光缀合物; 其中所述第一荧光共轭物选择性地与所述第一标签相关联。 所述探针还包括与靶材料结合的第二肽标签; 以及包含第二荧光染料的第二荧光缀合物，其具有来自第一荧光染料和第二标签结合基团的具有较长波长和不同激发和发射最大值的第二荧光染料。 在暴露于目标材料时，第一和第二荧光共轭物分别独立地与第一和第二肽标签相关联，以便间隔距离表示为约0.1倍R 0至约2倍 使得在激发第一荧光共轭物时，荧光共振能量转移导致第二荧光偶联物的激发，产生来自第二荧光缀合物的可检测的发射。