公开(公告)号：US08336367B2

公开(公告)日：2012-12-25

申请号：US12555221

申请日：2009-09-08

申请人： Jian Lu ,  Tsuyoshi Ikehara ,  Takashi Mihara

发明人： Jian Lu ,  Tsuyoshi Ikehara ,  Takashi Mihara

IPC分类号： G01N29/02

CPC分类号： G01G3/165

摘要： An object of the present invention is to provide a detection sensor and a vibrator which can offer improved sensitivity. The present invention uses a scheme in which no piezoelectric layer, drive electrode, or the like is provided on the surface of a vibrator 30 and in which the vibrator 30 is driven by an actuator 40 provided separately from the vibrator 30. The actuator 40 is of a cantilever type and is provided near a fixed end 30a of the vibrator 30. Vibration of the actuator 40 is transmitted to the vibrator 30 via a coupling beam 70. Thus, vibration of the vibrator 30 is prevented from being inhibited by a piezoelectric layer, a drive electrode, or the like. This improves the Q value of the vibrator 30 and thus the sensitivity of the detection sensor 10. Furthermore, if the detection sensor 10 is configured to offer a sensitivity equivalent to that of a conventional one, the size of the detection sensor 10 can be sharply reduced compared to that of the conventional one.

摘要翻译： 本发明的目的是提供一种能够提供更高灵敏度的检测传感器和振动器。 本发明使用在振动器30的表面上没有设置压电层，驱动电极等的方案，并且振动器30由与振动器30分开设置的致动器40驱动。致动器40是 并且设置在振动器30的固定端30a附近。致动器40的振动通过耦合梁70传递到振动器30.因此，防止振动器30的振动受到压电层的抑制 ，驱动电极等。 这提高了振动器30的Q值，从而提高了检测传感器10的灵敏度。此外，如果检测传感器10被配置为提供与常规灵敏度相当的灵敏度，则检测传感器10的尺寸可以是尖锐的 与传统方法相比有所减少。

公开(公告)号：US07745191B2

公开(公告)日：2010-06-29

申请号：US12094067

申请日：2005-11-16

申请人： Takeshi Yasumoto ,  Tsuyoshi Ikehara ,  Fukiko Shinjyo

发明人： Takeshi Yasumoto ,  Tsuyoshi Ikehara ,  Fukiko Shinjyo

IPC分类号： C12N9/16

CPC分类号： C12N9/16

摘要： The purpose of the invention is to provide an activated protein phosphatase 2A (PP2A) in large quantities with high purity by a genetic engineering and to provide a method for producing a heterodimer derivative of PP2A which comprises infecting insect cultured cells with a baculovirus in which a cDNA encoding the catalytic subunit of PP2A carrying a first tag is integrated together with another baculovirus in which a cDNA encoding the A subunit of PP2A carrying a second tag is integrated, incubating the infected cells, disrupting the incubated cells to obtain a disrupted cell suspension, and then purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the first tag and another solid phase carrying a substance capable of binding to the second tag, characterized in that the insect cells infected with the baculovirus are incubated at a temperature of from 18 to 22° C.

摘要翻译： 本发明的目的是通过基因工程提供大量高纯度的活化蛋白磷酸酶2A（PP2A），并提供生产PP2A异二聚体衍生物的方法，其包括用杆状病毒感染昆虫培养的细胞，其中 编码携带第一标签的PP2A的催化亚基的cDNA与另一种杆状病毒整合在一起，其中编码携带第二标签的PP2A的A亚基的cDNA整合，孵育感染的细胞，破坏孵育的细胞以获得破坏的细胞悬浮液， 然后用携带能够结合第一标签的物质的固相和另一种携带能够结合第二标签的物质的固相纯化破碎的细胞悬浮液，其特征在于用杆状病毒感染的昆虫细胞在 温度为18〜22℃

公开(公告)号：US20090093018A1

公开(公告)日：2009-04-09

申请号：US12094067

申请日：2005-11-16

申请人： Takeshi Yasumoto ,  Tsuyoshi Ikehara ,  Fukiko Shinjyo

发明人： Takeshi Yasumoto ,  Tsuyoshi Ikehara ,  Fukiko Shinjyo

IPC分类号： C12P21/00

CPC分类号： C12N9/16

摘要： The purpose of the invention is to provide an activated protein phosphatase 2A (PP2A) in large quantities with high purity by a genetic engineering and to provide a method for producing a heterodimer derivative of PP2A which comprises infecting insect cultured cells with a baculovirus in which a cDNA encoding the catalytic subunit of PP2A carrying a first tag is integrated together with another baculovirus in which a cDNA encoding the A subunit of PP2A carrying a second tag is integrated, incubating the infected cells, disrupting the incubated cells to obtain a disrupted cell suspension, and then purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the first tag and another solid phase carrying a substance capable of binding to the second tag, characterized in that the insect cells infected with the baculovirus are incubated at a temperature of from 18 to 22° C.

摘要翻译： 本发明的目的是通过基因工程提供大量高纯度的活化蛋白磷酸酶2A（PP2A），并提供生产PP2A异二聚体衍生物的方法，其包括用杆状病毒感染昆虫培养的细胞，其中 编码携带第一标签的PP2A的催化亚基的cDNA与另一种杆状病毒整合在一起，其中编码携带第二标签的PP2A的A亚基的cDNA整合，孵育感染的细胞，破坏孵育的细胞以获得破坏的细胞悬浮液， 然后用携带能够结合第一标签的物质的固相和另一种携带能够结合第二标签的物质的固相纯化破碎的细胞悬浮液，其特征在于用杆状病毒感染的昆虫细胞在 温度为18〜22℃