公开(公告)号：US20110282051A1

公开(公告)日：2011-11-17

申请号：US12892561

申请日：2010-09-28

申请人： Nancy E. Harding ,  Yamini N. Patel ,  Russell Coleman ,  Steven Matzke

发明人： Nancy E. Harding ,  Yamini N. Patel ,  Russell Coleman ,  Steven Matzke

IPC分类号： C08B37/00

CPC分类号： C12N1/20 ,  A61K9/4816 ,  A61K9/7007 ,  A61K47/38 ,  C08B11/12 ,  C08B37/0033 ,  C08L1/286 ,  C12N9/10 ,  C12P19/04 ,  C12P19/06

摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.

摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

公开(公告)号：US20080319186A1

公开(公告)日：2008-12-25

申请号：US11264268

申请日：2005-11-01

申请人： Nancy E. Harding ,  Yamini N. Patel ,  Russell Coleman ,  Steven Matzke

发明人： Nancy E. Harding ,  Yamini N. Patel ,  Russell Coleman ,  Steven Matzke

IPC分类号： C12P19/04

CPC分类号： C12N1/20 ,  A61K9/4816 ,  A61K9/7007 ,  A61K47/38 ,  C08B11/12 ,  C08B37/0033 ,  C08L1/286 ,  C12N9/10 ,  C12P19/04 ,  C12P19/06

摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.

摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

公开(公告)号：US06764408B1

公开(公告)日：2004-07-20

申请号：US10290480

申请日：2002-11-05

申请人： Russell Coleman

发明人： Russell Coleman

IPC分类号： A63G31112

CPC分类号： A63G31/12 ,  A63G21/22

摘要： An inflatable game incorporating a flexible, inflated structure filled with gas, suitable for users to jump and bounce thereupon and a suspended structure that permits users to be suspended above the inflatable structure. The suspended structure may be a zip line, a bridge, or the like. The suspended structure allows users to move from one elevated position on the inflatable structure to another elevated position. The inflatable structures used in conjunction with suspended features provide an added variety of activities to the user. For example, users can hang in midair, drop onto the inflatable structure from elevated heights, engage in competitive activities.

摘要翻译： 一种充气游戏，包含充满气体的柔性膨胀结构，适合使用者跳跃和弹跳，并且悬挂结构允许使用者悬挂在充气结构之上。 悬挂结构可以是拉链线，桥等。 悬挂结构允许用户从充气结构上的一个提升位置移动到另一个提升位置。 与悬挂特征结合使用的充气结构为用户提供了多种活动。 例如，用户可以悬挂在空中，从高处悬挂在充气结构上，从事竞争活动。