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公开(公告)号：US09869166B2
公开(公告)日：2018-01-16
申请号：US14917871
申请日：2014-09-11
CPC分类号： E21B43/20 , C09K8/582 , C12N1/26 , C12P7/6463 , C12P19/06
摘要： The present invention provides methods for increasing the viscosity of the drive fluid for displacing oil from a subterranean formation by the use of microorganisms selected or modified for the ability to produce cell free polymers without the formation of any significant bioplugging biofilm or capsule.
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公开(公告)号：US20150125932A1
公开(公告)日：2015-05-07
申请号：US14585094
申请日：2014-12-29
申请人： CP Kelco U.S., Inc.
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
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公开(公告)号：US08278438B2
公开(公告)日：2012-10-02
申请号：US12892561
申请日：2010-09-28
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
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公开(公告)号：US20110282051A1
公开(公告)日：2011-11-17
申请号：US12892561
申请日：2010-09-28
IPC分类号： C08B37/00
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
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公开(公告)号：US20080319186A1
公开(公告)日：2008-12-25
申请号：US11264268
申请日：2005-11-01
IPC分类号： C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
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6.发明授权Isolated gum operon from Xyllela fastidiosa, isolated nucleic acid molecules therefrom, and uses thereof 失效来自樟树的分离的口香糖操纵子，其分离的核酸分子及其用途公开(公告)号：US07285409B1
公开(公告)日：2007-10-23
申请号：US09567465
申请日：2000-05-09
申请人： Paulo Arruda , Felipe Rodrigues da Silva , Edson Luis Kemper , Andre Vettorc , Adilson Leite , Marcio Jose da Silva
发明人： Paulo Arruda , Felipe Rodrigues da Silva , Edson Luis Kemper , Andre Vettorc , Adilson Leite , Marcio Jose da Silva
CPC分类号： C12N15/52 , C07K14/195 , C12N9/1029 , C12N9/1048 , C12N9/1051 , C12P19/06
摘要： This invention relates to the isolation and characterization of an operon from the organism Xylella fastidiosa. This operon, referred to as the XfGUM operon, contains 9 genes, and is involved in the production of a compound with xanthan gum like properties. The expression products of the operon and genes are also a part of the invention, as are various uses of these.
摘要翻译： 本发明涉及从生物X螨生物中分离和表征操纵子。 该操纵子称为XfGUM操纵子，其中包含9个基因，并且涉及生产具有黄原胶的化合物。 操纵子和基因的表达产物也是本发明的一部分，以及这些的各种用途。
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7.发明申请Genetic control of acetylation and pyruvylation of xanthan based polysaccharide polymers 审中-公开黄原胶多糖聚合物的乙酰化和丙酮酸化的遗传控制公开(公告)号：US20020103370A1
公开(公告)日：2002-08-01
申请号：US09986803
申请日：2001-11-13
发明人： Daniel H. Doherty , Donna M. Ferber , John D. Marrelli , Rebecca W. Vanderslice , Randal A. Hassler
IPC分类号： C07H013/02
CPC分类号： C12R1/64 , C08B37/0033 , C09K8/08 , C12N15/00 , C12N15/01 , C12P19/06 , Y10S435/822
摘要： Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta-null1,4null configuration. The inner D-mannose moieties are linked in an alpha-null1,3null configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta-null1,2null configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta-null1,4null configuration. Processes for preparing the polysaccharide polymers are also provided.
摘要翻译： 提供变体黄原胶，其包括水溶性多糖聚合物，其包含具有约2:2:1的D-葡萄糖：D-甘露糖：D-葡萄糖醛酸比率的重复五聚体单元和包含重复四聚体的水溶性多糖聚合物 具有D-葡萄糖：D-甘露糖：D-葡萄糖醛酸比例为约2:1:1的单元。 D-葡萄糖部分以β-（1,4）构型连接。 内部D-甘露糖部分以α-[1,3]构型连接，通常为交替的葡萄糖部分。 D-葡萄糖醛酸部分以β-（1,2）构型连接到内部甘露糖部分。 外部甘露糖部分以β-（1,4）构型与葡萄糖醛酸部分连接。 还提供了制备多糖聚合物的方法。
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公开(公告)号：US06365207B2
公开(公告)日：2002-04-02
申请号：US09834510
申请日：2001-04-13
申请人： Craig Alan Hoppe , Jeanette Lawrence , Amr Shaheed
发明人： Craig Alan Hoppe , Jeanette Lawrence , Amr Shaheed
IPC分类号： C12P1906
CPC分类号： A23G3/366 , A23G9/363 , A23L23/00 , A23L27/60 , A23L29/27 , A23L29/273 , A61K47/36 , C12P19/06
摘要： A food or pharmaceutical composition including a liquid composition comprising the liquid fermentation broth product of a biologically active substance, in a carbohydrate medium other than dairy whey wherein said liquid fermentation broth product has not been subject to any concentrating or drying steps prior to introduction of the broth product into said food or pharmaceutical composition is provided.
摘要翻译： 一种食品或药物组合物，其包含液体组合物，其包含生物活性物质的液体发酵肉汤产物，在除乳制乳清之外的碳水化合物培养基中，其中所述液体发酵肉汤产品在引入之前未经过任何浓缩或干燥步骤 将肉汤产品加入到所述食品或药物组合物中。
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9.发明申请Process for clarification of xanthan solutions and xanthan gum produced thereby 失效由此生产的黄原胶和黄原胶的澄清方法公开(公告)号：US20020031525A1
公开(公告)日：2002-03-14
申请号：US09833559
申请日：2001-04-13
IPC分类号： A61K031/00 , C12P019/04
CPC分类号： C12Y302/01017 , A23L29/27 , A61K2800/51 , C08B37/0003 , C08B37/0033 , C12P19/06 , C12Y304/00
摘要： A process is disclosed for clarifying an aqueous xanthan gum solution comprising treatment of the xanthan solution with at least one chelating agent, surfactant, organic acid, or a mixture thereof, and with a protease enzyme or a lysozyme and a protease enzyme. Also disclosed is a highly purified solid xanthan gum and compositions containing the same, wherein the gum is obtained from the clarified xanthan gum solution.
摘要翻译： 公开了一种用于澄清黄原胶水溶液溶液的方法，包括用至少一种螯合剂，表面活性剂，有机酸或其混合物以及蛋白酶或溶菌酶和蛋白酶进行黄原胶溶液的处理。 还公开了高纯度的固体黄原胶和含有该固体黄原胶的组合物，其中胶从澄清的黄原胶溶液获得。
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公开(公告)号：US06110731A
公开(公告)日：2000-08-29
申请号：US206414
申请日：1998-12-07
申请人： Kanji Murofushi , Shigehiro Nagura , Taira Homma
发明人： Kanji Murofushi , Shigehiro Nagura , Taira Homma
CPC分类号： C12M27/08 , B01F7/00241 , B01F7/00283 , B01F7/00341 , B01F7/00633 , B01F7/00641 , B01F7/00691 , C12P19/06 , C12P19/10
摘要： A fermenter for the production of xanthan gum is provided with an upper helical impeller and a lower turbine impeller which are positioned therewithin, and an agitator shaft for driving these helical impeller and turbine impeller. The helical impeller consists of a pair of vertically spaced arms extending from the agitator shaft in opposite directions and arranged in twisted relationship, and at least one shearing paddle bridging these arms, and the turbine impeller consists of a rotating disc having at least one turbine blade attached thereto.
摘要翻译： 用于生产黄原胶的发酵罐设置有定位在其中的上螺旋叶轮和下涡轮叶轮，以及用于驱动这些螺旋叶轮和涡轮叶轮的搅拌轴。 螺旋叶轮包括一对垂直间隔开的臂，其从相反方向从搅拌器轴延伸并且以扭曲关系布置，并且至少一个桥接这些臂的剪切桨，涡轮叶轮由具有至少一个涡轮叶片 附在其上。
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