-
1.发明申请Isolated Nucleic Acids for Producing High Viscosity Diutan Gums and Methods 审中-公开用于生产高粘度Diutan Gums和方法的分离核酸公开(公告)号：US20130059363A1
公开(公告)日：2013-03-07
申请号：US13599210
申请日：2012-08-30
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US20130084607A1
公开(公告)日：2013-04-04
申请号：US13599202
申请日：2012-08-30
IPC分类号： C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US07868167B2
公开(公告)日：2011-01-11
申请号：US11264268
申请日：2005-11-01
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US08921077B2
公开(公告)日：2014-12-30
申请号：US13599202
申请日：2012-08-30
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US08278438B2
公开(公告)日：2012-10-02
申请号：US12892561
申请日：2010-09-28
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US20110282051A1
公开(公告)日：2011-11-17
申请号：US12892561
申请日：2010-09-28
IPC分类号： C08B37/00
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
公开(公告)号：US20080319186A1
公开(公告)日：2008-12-25
申请号：US11264268
申请日：2005-11-01
IPC分类号： C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译： 与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。 这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。 质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。 以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。 这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。 本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。 此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.022 秒)
