公开(公告)号：US20210139876A1

公开(公告)日：2021-05-13

申请号：US17148790

申请日：2021-01-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20190352629A1

公开(公告)日：2019-11-21

申请号：US16318496

申请日：2017-07-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben Peter Friis ,  Jens Erik Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US09828595B2

公开(公告)日：2017-11-28

申请号：US14420399

申请日：2013-08-15

Applicant: Novozymes A/S

Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

IPC: C12N9/82 ,  A23L5/20

CPC classification number: C12N9/82 ,  A23L5/25 ,  C12Y305/01001

Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.