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    Thermostable Asparaginase Variants and Polynucleotides Encoding Same
    3.
    发明申请
    Thermostable Asparaginase Variants and Polynucleotides Encoding Same 有权
    恒温天冬酰胺酶变体和编码相同的多核苷酸

    公开(公告)号：US20150210995A1

    公开(公告)日：2015-07-30

    申请号：US14420399

    申请日：2013-08-15

    Applicant: NOVOZYMES A/S

    Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

    IPC: C12N9/82

    CPC classification number: C12N9/82 ,  A23L5/25 ,  C12Y305/01001

    Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

    Abstract translation: 本发明涉及天冬酰胺酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。 本发明进一步涉及一种生产发酵产物的方法，包括：在本发明的天冬酰胺酶存在下，将含淀粉的物质用α-淀粉酶液化; 将糊精糖化成糖与葡糖淀粉酶; 并使用发酵生物发酵糖。

    Polypeptides Having Pullulanase Activity Suitable For Use In Liquefaction
    4.
    发明申请
    Polypeptides Having Pullulanase Activity Suitable For Use In Liquefaction 有权

    公开(公告)号：US20220154226A1

    公开(公告)日：2022-05-19

    申请号：US17440477

    申请日：2020-03-17

    Applicant: Novozymes A/S

    Inventor： Takashi Nakanishi ,  Aki Tomiki ,  Yuma Kurakata

    IPC: C12P7/06

    Abstract: The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

    Serine protease variants and polynucleotides encoding same
    5.
    发明授权
    Serine protease variants and polynucleotides encoding same 有权

    公开(公告)号：US10927361B2

    公开(公告)日：2021-02-23

    申请号：US16318496

    申请日：2017-07-14

    Applicant: NOVOZYMES A/S

    Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

    IPC: C12N9/58 ,  C12P7/06

    Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    Polypeptides having pullulanase activity suitable for use in liquefaction
    7.
    发明授权
    Polypeptides having pullulanase activity suitable for use in liquefaction 有权

    公开(公告)号：US11427811B2

    公开(公告)日：2022-08-30

    申请号：US15746162

    申请日：2016-07-11

    Applicant: NOVOZYMES A/S

    Inventor： Tomoko Matsui ,  Suzanne Clark ,  Aki Tomiki

    IPC: C12N9/44 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  C12C5/00 ,  C12C7/04 ,  C12N9/34 ,  C13K1/06

    Abstract: The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.

    Serine protease variants and polynucleotides encoding same
    10.
    发明授权
    Serine protease variants and polynucleotides encoding same 有权

    公开(公告)号：US11447763B2

    公开(公告)日：2022-09-20

    申请号：US17148790

    申请日：2021-01-14

    Applicant: NOVOZYMES A/S

    Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

    IPC: C12N9/58 ,  C12P7/06

    Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

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