公开(公告)号：US12005101B2

公开(公告)日：2024-06-11

申请号：US17515001

申请日：2021-10-29

申请人： PEMI31 Therapeutics Inc.

发明人： Edward R. M. O'Brien ,  Chunhua Shi ,  Yong-Xiang Chen

IPC分类号： A61K39/00 ,  A61K38/17 ,  A61K39/39 ,  A61K39/395 ,  A61P3/06 ,  C07K14/47 ,  C07K16/18 ,  C12N9/58 ,  G01N33/543

CPC分类号： A61K39/0005 ,  A61K38/1709 ,  A61K39/39 ,  A61K39/3955 ,  A61P3/06 ,  C07K14/47 ,  C07K14/4703 ,  C07K16/18 ,  C12N9/58 ,  G01N33/54326 ,  A61K39/395 ,  A61K2039/55505 ,  A61K2039/55566 ,  G01N2333/4703

摘要： This disclosure pertains to compositions and methods for reducing serum cholesterol in mammalian subjects. The exemplary compositions comprise mixtures of HSP27 protein or fragments thereof, a HSP25 protein or fraction thereof, a recombinant rHSP25 peptide, or a recombinant HSP27 peptide in a mixture with an adjuvant. The compositions may optionally comprise anti-HSP27 antibody. The methods for reducing serum cholesterol in mammalian subjects relate to the use of the compositions to increase the subjects' levels of serum HSP27 and/or anti-HSP27 antibodies.

公开(公告)号：US20220307036A1

公开(公告)日：2022-09-29

申请号：US17615807

申请日：2020-07-24

申请人： Novozymes A/S

发明人： Monica Tassone ,  Judilee Osborn

IPC分类号： C12N15/62 ,  C12P21/02 ,  C12N9/30 ,  C12N9/34 ,  C12N9/42 ,  C12N9/36 ,  C12N9/52 ,  C12N9/58 ,  C12P7/10

摘要： Described herein are recombinant host organisms expressing a fusion protein with a foreign signal linked to the N-terminus of a mature polypeptide, such as an alpha-amylase, protease, beta-glucosidase or glucoamylase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant host organisms.

公开(公告)号：US11447763B2

公开(公告)日：2022-09-20

申请号：US17148790

申请日：2021-01-14

申请人： NOVOZYMES A/S

发明人： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC分类号： C12N9/58 ,  C12P7/06

摘要： The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20220290165A1

公开(公告)日：2022-09-15

申请号：US17529931

申请日：2021-11-18

申请人： GLYKOS FINLAND OY

发明人： Christopher LANDOWSKI ,  Anne HUUSKONEN ,  Juhani SAARINEN ,  Ann WESTERHOLM-PARVINEN ,  Anne KANERVA ,  Jari NATUNEN ,  Anna-Liisa HÄNNINEN ,  Noora SALOVUORI ,  Merja PENTTILÄ ,  Markku SALOHEIMO

IPC分类号： C12N15/80 ,  C12P21/00 ,  C12P21/02 ,  C12N9/58 ,  C07K16/00 ,  C07K16/28 ,  C07K14/56 ,  C07K14/61 ,  C07K14/65

摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

公开(公告)号：US11236317B2

公开(公告)日：2022-02-01

申请号：US16067357

申请日：2017-02-04

申请人： NOVOZYMES A/S

发明人： Ye Liu ,  Xianzhi Jiang ,  Lan Tang ,  Astrid Benie ,  Henrik Frisner ,  Bucong Han

IPC分类号： C12N9/58 ,  C11D3/386

摘要： Disclosed are isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. Nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides are also disclosed.

公开(公告)号：US11155798B2

公开(公告)日：2021-10-26

申请号：US16567167

申请日：2019-09-11

申请人： NOVOZYMES A/S

发明人： Joseph Jump ,  Nathaniel Kreel ,  Jeremy Saunders ,  Michael John Akerman

IPC分类号： C12P7/06 ,  C12P7/14 ,  C12N9/24 ,  C12N9/50 ,  C12N9/48 ,  C12N9/30 ,  C12N9/42 ,  C12N9/58 ,  C12P19/14 ,  C13K1/06 ,  C12P19/02 ,  C12N9/28 ,  C12N9/34

摘要： The invention relates to enzyme compositions comprising a glucoamylase, an alpha-amylase, and optionally a cellulolytic composition and/or a protease. The invention also relates to the use thereof in processes of producing sugars and/or fermentation products from starch-containing material by saccharifying and/or fermenting starch-containing material at a temperature below the initial gelatinization temperature.

公开(公告)号：US11078510B2

公开(公告)日：2021-08-03

申请号：US16996168

申请日：2020-08-18

申请人： Novozymes, Inc.

发明人： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC分类号： C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N15/04 ,  C12N9/58

摘要： The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20210139876A1

公开(公告)日：2021-05-13

申请号：US17148790

申请日：2021-01-14

申请人： NOVOZYMES A/S

发明人： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC分类号： C12N9/58 ,  C12P7/06

摘要： The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10851362B2

公开(公告)日：2020-12-01

申请号：US16027768

申请日：2018-07-05

申请人： AGRIVIDA, INC.

发明人： R. Michael Raab ,  Binzhang Shen ,  Gabor Lazar ,  Humberto De La Vega

IPC分类号： C12N1/21 ,  C12N9/14 ,  C12N15/57 ,  C07K19/00 ,  A23K20/189 ,  C12N9/52 ,  C12N9/64 ,  C07K14/81 ,  C12N15/62 ,  C12N15/63 ,  C12N9/50 ,  C12N9/20 ,  C12N9/42 ,  C12N9/24 ,  C12N9/54 ,  C11D3/386 ,  C12N9/58 ,  C12N9/62

摘要： Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.

公开(公告)号：US10829598B2

公开(公告)日：2020-11-10

申请号：US15220159

申请日：2016-07-26

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS ,  UNIVERSITE DE POITIERS ,  VALAGRO CARBONE RENOUVELABLE POITOU-CHARENTES

发明人： Thierry Ferreira ,  Frederic Bataille ,  Cedric Dever ,  Jacques Barbier

IPC分类号： C08J3/00 ,  C08J3/20 ,  C12N9/14 ,  C12N11/04 ,  C08L67/04 ,  C12N9/20 ,  C12N9/58 ,  C12N11/18 ,  C12N11/08 ,  C08K5/00

摘要： The present invention relates to a process for preparing a polymer/biological entities alloy, comprising a step of mixing a polymer and biological entities that degrade it, during a heat treatment, said heat treatment being performed at a temperature T above room temperature and said biological entities being resistant to said temperature T, characterized in that said biological entities are chosen from enzymes that degrade said polymer and microorganisms that degrade said polymer.