公开(公告)号：US11913944B2

公开(公告)日：2024-02-27

申请号：US16825802

申请日：2020-03-20

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Robin Hurst ,  Thomas Kirkland ,  Keith Wood

IPC分类号： G01N33/533 ,  G01N21/64 ,  G01N21/76 ,  G01N33/542

CPC分类号： G01N33/533 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/763 ,  G01N33/542 ,  G01N2021/6439

摘要： Provided herein are compositions and methods for photoaffinity labeling of molecular targets. In particular, probes that specifically interact with cellular targets based on their affinity and are then covalently linked to the cellular target via a photoreactive group (PRG) on the probe.

公开(公告)号：US11667648B2

公开(公告)日：2023-06-06

申请号：US16813295

申请日：2020-03-09

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Thomas Kirkland ,  Keith V. Wood

IPC分类号： C07D491/22 ,  C07D231/40 ,  C07D487/04

CPC分类号： C07D491/22 ,  C07D231/40 ,  C07D487/04

摘要： Provided herein are cell-permeable, cell-compatible, and chemoselectively-cleavable linkers for tethering (e.g., covalently) functional elements (e.g., a cellular interaction element and a capture element), and methods of use (e.g., intracellular capture and extracellular release of cellular targets) therewith.

公开(公告)号：US20140322794A1

公开(公告)日：2014-10-30

申请号：US14207993

申请日：2014-03-13

申请人： PROMEGA CORPORATION

发明人： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC分类号： C12N9/14 ,  C12Q1/34

CPC分类号： C12Q1/34 ,  C12N9/14 ,  G01N33/54353

摘要： The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.