公开(公告)号：US11534504B2

公开(公告)日：2022-12-27

申请号：US16592310

申请日：2019-10-03

申请人： Promega Corporation

发明人： Melanie Dart ,  Thomas Smith ,  Thomas Kirkland ,  Thomas Machleidt ,  Keith Wood

IPC分类号： A61K49/00 ,  C07D487/04 ,  G01N33/58

摘要： Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.

公开(公告)号：US20220348983A1

公开(公告)日：2022-11-03

申请号：US17332459

申请日：2021-05-27

申请人： Promega Corporation

发明人： Brock Binkowski ,  Lance P. Encell ,  Mary Hall ,  Matthew B. Robers ,  Michael R. Slater ,  Keith V. Wood ,  Monika G. Wood

IPC分类号： C12Q1/66 ,  C07D487/04 ,  C12N9/02 ,  C12N15/52

摘要： An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.

公开(公告)号：US11214822B2

公开(公告)日：2022-01-04

申请号：US16672090

申请日：2019-11-01

申请人： PROMEGA CORPORATION

发明人： Michael P. Valley ,  James J. Cali ,  Brock Binkowski ,  Christopher Todd Eggers ,  Keith V. Wood

IPC分类号： C12Q1/66 ,  C12Q1/26

摘要： Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.

公开(公告)号：US20210403886A9

公开(公告)日：2021-12-30

申请号：US15980406

申请日：2018-05-15

申请人： Promega Corporation

发明人： Andrew S. Dixon ,  Lance P. Encell ,  Thomas Machleidt ,  Marie Schwinn ,  Keith Wood ,  Monika Wood ,  Kris Zimmerman

IPC分类号： C12N9/14 ,  G01N33/535 ,  G01N33/533 ,  C07K14/315 ,  C07K14/475

摘要： Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

公开(公告)号：US11175228B2

公开(公告)日：2021-11-16

申请号：US16698143

申请日：2019-11-27

申请人： Promega Corporation

发明人： Hui Wang ,  Nidhi Nath ,  Rod Flemming ,  Becky Godat ,  Wenhui Zhou ,  Virginia Kincaid ,  Melanie Dart

IPC分类号： G01N21/64 ,  C07K7/08 ,  G01N33/542 ,  C07K1/13 ,  C07K14/00 ,  C07D207/46 ,  C07K16/24

摘要： Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.

公开(公告)号：US11164144B2

公开(公告)日：2021-11-02

申请号：US16265608

申请日：2019-02-01

申请人： PROMEGA CORPORATION

发明人： Mark Sehmer ,  Travis Phillips ,  Mike Lohmeier

IPC分类号： G06Q10/08 ,  G06K7/10 ,  H04W4/80 ,  G06F21/35 ,  G06K19/077 ,  H04L29/08 ,  G06F9/50

摘要： A multi-tenant, RFID system that may be Cloud based or run on a local area network (LAN) for distributed RFID devices and RFID applications. The RFID system a central abstraction and translation layer between RFID devices installed in geographically diverse locations and applications. RFID devices initiate communication to a Cloud or LAN network over to send events and receive commands. RFID applications can receive RFID tag data, device health, and requested and derived events from the RFID system to automatically run processes based on the provided data. Applications manage RFID devices and settings in the RFID system using command and configuration interfaces.

公开(公告)号：US20210262941A1

公开(公告)日：2021-08-26

申请号：US17105925

申请日：2020-11-27

申请人： Promega Corporation

发明人： Virginia Kincaid ,  Lance P. Encell ,  Mary Hall ,  Michael Killoran ,  Keith Wood ,  Melanie Dart ,  Trish Hoang

IPC分类号： G01N21/76 ,  C12N9/02 ,  G01N33/58

摘要： Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.

公开(公告)号：US10976312B2

公开(公告)日：2021-04-13

申请号：US15402879

申请日：2017-01-10

申请人： Promega Corporation

发明人： Carolyn W. Hitko ,  Robin Hurst ,  Thomas Kirkland ,  Dieter Klaubert ,  Poncho Meisenheimer ,  Nidhi Nath ,  Rachel Friedman Ohana ,  Paul Otto ,  Marjeta Urh ,  Harry Tetsuo Uyeda ,  Keith Wood ,  Ji Zhu

IPC分类号： G01N33/543

摘要： The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

公开(公告)号：US10590162B2

公开(公告)日：2020-03-17

申请号：US15322519

申请日：2015-07-02

申请人： PROMEGA CORPORATION

发明人： Thomas Kirkland ,  Mark McDougall ,  Poncho Meisenheimer ,  Min Zhou

IPC分类号： C07H21/04 ,  C12Q1/6816 ,  C07H21/00 ,  C12N9/12 ,  C12Q1/6848 ,  C12Q1/686

摘要： Provided herein are chelator constructs (e.g., nucleic acid, peptide, peptide nucleic acid, etc.) that sequester metal ions (e.g., Mg2+) under a first set of conditions and fail to sequester or release sequestered metal ions under a second set of conditions. In particular, nucleic acid constructs are provided that sequester metal ions (e.g., Mg2+) under conditions that favor secondary and tertiary structure formation and release or fail to sequester metal ions under conditions that disfavor the formation of such structures.

公开(公告)号：US10513694B2

公开(公告)日：2019-12-24

申请号：US15192420

申请日：2016-06-24

申请人： PROMEGA CORPORATION

发明人： Sarah Duellman ,  Matthew B. Robers ,  Joel R. Walker ,  Wenhui Zhou ,  Chad Zimprich

IPC分类号： C07D493/04 ,  C07D495/04 ,  C07D209/42 ,  C12N9/02 ,  C12Q1/66 ,  G01N33/58 ,  C07D207/34 ,  C07D491/048 ,  C07K5/093 ,  G01N33/542 ,  C07K5/02

摘要： Thienopyrrole compounds that may inhibit Oplophorus-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.