公开(公告)号：US10882035B2

公开(公告)日：2021-01-05

申请号：US15591842

申请日：2017-05-10

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Thomas Kirkland ,  Keith Wood

IPC分类号： B01J31/24 ,  C07B63/04 ,  B01J31/28 ,  C07F15/00

摘要： Provided herein are palladium (Pd) catalysts with improved performance in biological environments. In particular, formulations, methods of preparations, and storage conditions are provided that provide improved performance of Pd catalysts under protein-rich conditions.

公开(公告)号：US20160355523A1

公开(公告)日：2016-12-08

申请号：US15172860

申请日：2016-06-03

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Thomas Kirkland ,  Keith V. Wood

IPC分类号： C07D491/22 ,  C07D487/04 ,  G01N33/573 ,  C07D231/40

CPC分类号： C07D491/22 ,  C07D231/40 ,  C07D487/04

摘要： Provided herein are cell-permeable, cell-compatible, and chemoselectively-cleavable linkers for tethering (e.g., covalently) functional elements (e.g., a cellular interaction element and a capture element), and methods of use (e.g., intracellular capture and extracellular release of cellular targets) therewith.

摘要翻译： 本文提供了用于系链（例如，共价）功能元件（例如，细胞相互作用元件和捕获元件）的细胞可渗透，细胞相容性和化学选择性可切割的接头，以及使用方法（例如，细胞内捕获和细胞外释放 的细胞靶）。

公开(公告)号：US20210340593A1

公开(公告)日：2021-11-04

申请号：US17374460

申请日：2021-07-13

申请人： Promega Corporation

发明人： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC分类号： C12Q1/34 ,  G01N33/543 ,  C12N9/14

摘要： The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

公开(公告)号：US20170096560A1

公开(公告)日：2017-04-06

申请号：US15282590

申请日：2016-09-30

申请人： PROMEGA CORPORATION

发明人： Stephen J. Dwight ,  Sergiy Levin

IPC分类号： C09B11/24

CPC分类号： C09B11/24 ,  C07D311/82 ,  C07D493/14 ,  C07D493/22

摘要： Described herein are methods for the regioselective synthesis of substituted rhodamine dyes.

公开(公告)号：US20200340982A1

公开(公告)日：2020-10-29

申请号：US16825802

申请日：2020-03-20

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Robin Hurst ,  Thomas Kirkland ,  Keith Wood

IPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/542 ,  G01N21/64

摘要： Provided herein are compositions and methods for photoaffinity labeling of molecular targets. In particular, probes that specifically interact with cellular targets based on their affinity and are then covalently linked to the cellular target via a photoreactive group (PRG) on the probe.

公开(公告)号：US10093806B2

公开(公告)日：2018-10-09

申请号：US15282590

申请日：2016-09-30

申请人： PROMEGA CORPORATION

发明人： Stephen J. Dwight ,  Sergiy Levin

IPC分类号： C09B11/24

摘要： Described herein are methods for the regioselective synthesis of substituted rhodamine dyes.

公开(公告)号：US20140322738A1

公开(公告)日：2014-10-30

申请号：US14207959

申请日：2014-03-13

申请人： PROMEGA CORPORATION

发明人： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC分类号： C12Q1/34

CPC分类号： C12Q1/34 ,  C12N9/14 ,  G01N33/54353

摘要： The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

摘要翻译： 本发明提供了卤代烷烃基材和用于将这些基材连接到功能元件（例如标签，标签，表面等）上的连接器。 本文所述的底物和接头可用于例如蛋白质，细胞和分子的标记，检测和固定。 特别地，本文提供的接头在底物中用于与其卤代烷基底物形成共价键的脱卤酶变体。

公开(公告)号：US11939311B2

公开(公告)日：2024-03-26

申请号：US16986682

申请日：2020-08-06

申请人： Promega Corporation

发明人： Joel Walker ,  Sergei Saveliev ,  Zhiyang Zeng ,  Robert Kargbo ,  Hui Wang ,  Sergiy Levin ,  Valerie Ressler ,  Jun Zhang ,  Ce Shi ,  Harry Tetsuo Uyeda ,  Jean Osterman ,  Min Zhou ,  Prabin Rai ,  Wenhui Zhou

IPC分类号： C07D317/24 ,  C07C309/15 ,  C12Q1/37 ,  G01N33/68

CPC分类号： C07D317/24 ,  C07C309/15 ,  C12Q1/37 ,  G01N33/6848

摘要： Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

公开(公告)号：US20210040058A1

公开(公告)日：2021-02-11

申请号：US16986682

申请日：2020-08-06

申请人： Promega Corporation

发明人： Joel Walker ,  Sergei Saveliev ,  Zhiyang Zeng ,  Robert Kargbo ,  Hui Wang ,  Sergiy Levin ,  Valerie Ressler ,  Jun Zhang ,  Ce Shi ,  Harry Tetsuo Uyeda ,  Jean Osterman ,  Min Zhou ,  Prabin Rai ,  Wenhui Zhou

IPC分类号： C07D317/24 ,  C07C309/15 ,  C07C309/14 ,  C12Q1/37 ,  G01N33/68

摘要： Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

公开(公告)号：US20180029028A1

公开(公告)日：2018-02-01

申请号：US15591842

申请日：2017-05-10

申请人： Promega Corporation

发明人： Sergiy Levin ,  Rachel Friedman Ohana ,  Thomas Kirkland ,  Keith Wood

IPC分类号： B01J31/24 ,  C07F15/00

CPC分类号： B01J31/2404 ,  B01J31/24 ,  B01J31/28 ,  B01J2231/005 ,  B01J2531/824 ,  B01J2540/225 ,  B01J2540/32 ,  C07B63/04 ,  C07F15/006 ,  C07F15/0066

摘要： Provided herein are palladium (Pd) catalysts with improved performance in biological environments. In particular, formulations, methods of preparations, and storage conditions are provided that provide improved performance of Pd catalysts under protein-rich conditions.